Evaluating the use of 3′-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions
Evaluating the use of 3′-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions
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DOI：
10.1186/1467-4866-10-8
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发表时间：
2009-08-11
影响因子：
2.3
通讯作者：
Schoonen, Martin A. A.
中科院分区：
文献类型：
--
作者：
Cohn, Corey A.;Pedigo, Christopher E.;Schoonen, Martin A. A.
背景：鉴于高活性氧（hROS）作为反应物在广泛的生物、光化学和环境系统中的重要性，人们对这些物种的检测和量化很感兴趣。hROS的极端反应性，其中包括羟基自由基，提出了分析的挑战。3′-（对氨基苯基）荧光素（APF）是一种用于测定hROS的较新的探针。在这里，我们进一步评估了使用APF作为检测颗粒悬浮液中羟基自由基的方法。结果：颗粒生成的hROS可定量测定，估计检出限为50 nM。在两种美国国家标准与技术研究所（NIST 2709和2710）土壤悬浮液和黄铁矿悬浮液中对hROS的测量显示出hROS产生的非线性颗粒剂量响应曲线。APF也可以用于不含溶解分子氧（O-2）的溶液中，以确定O-2在hROS形成中的作用。结果证实O-2在溶解的亚铁和黄铁矿悬浮液中形成hROS的机制上是重要的。结论：考虑到hROS颗粒生成的非线性剂量-响应曲线，我们建议在实验中使用几种颗粒负载，以比较颗粒的hROS生成潜力。本文提出的方法是针对hROS的，操作简单。分析可以在移动实验室进行，因为只需要基本的实验室设备。
Background: Given the importance of highly reactive oxygen species (hROS) as reactants in a wide range of biological, photochemical, and environmental systems there is an interest in detection and quantification of these species. The extreme reactivity of the hROS, which includes hydroxyl radicals, presents an analytical challenge. 3'-(p-Aminophenyl) fluorescein (APF) is a relatively new probe used for measuring hROS. Here, we further evaluate the use of APF as a method for the detection of hydroxyl radicals in particle suspensions.Results: Particle-generated hROS can be quantified with an estimated detection limit of 50 nM. Measurements of hROS in two National Institute of Standards and Technology (NIST 2709 and 2710) soil suspensions and a pyrite suspension show non-linear particle dose-response curves for hROS generation. APF can also be used in solutions containing no dissolved molecular oxygen (O-2) to determine the role of O-2 in the formation of hROS. Results confirm that O-2 is mechanistically important in the formation of hROS by dissolved ferrous iron and in pyrite suspensions.Conclusion: Given the non-linear dose-response curves for particle generation of hROS, we recommend using several particle loadings in experiments aimed to compare particles for their hROS generation potential. The method presented here is specific to hROS and simple to perform. The analysis can be conducted in mobile labs as only basic laboratory equipment is required.