THE MOLECULAR-BASIS OF I-R HYBRID DYSGENESIS IN DROSOPHILA-MELANOGASTER - IDENTIFICATION, CLONING, AND PROPERTIES OF THE I-FACTOR
THE MOLECULAR-BASIS OF I-R HYBRID DYSGENESIS IN DROSOPHILA-MELANOGASTER - IDENTIFICATION, CLONING, AND PROPERTIES OF THE I-FACTOR
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DOI：
10.1016/0092-8674(84)90536-1
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
FINNEGAN, DJ
中科院分区：
文献类型：
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作者：
BUCHETON, A;PARO, R;FINNEGAN, DJ
对白眼基因的两个突变进行了分析，这两个突变出现在患有I-R杂交发育不良的果蝇中。这些突变与插入明显相同的5.4千碱基序列有关，这些序列被克隆出来。这些插入显然是控制I-R杂交不育的I因子的副本。I因子不是可转座元件的拷贝样类或折叠类的成员，并且与控制P-M发育不全的P因子没有序列同源性。所有菌株的黑腹葡萄球菌都含有I因子序列。出现在反应性菌株中的那些必须代表不活跃的I元素。I元件在所有活性菌株中具有非常相似的序列组织，并且位于着丝粒周围区域。诱导菌株似乎既含有位于着丝粒周围区域的I元件，又含有位于染色体臂上的10-15个拷贝的完整I因子。
Two mutations of the white-eye gene, which arose in flies subject to I-R hybrid dysgenesis were analyzed. These mutations are associated with insertions of apparently identical 5.4 kilobase sequences, which were cloned. These insertions apparently are copies of the I factor controlling I-R hybrid dysgenesis. The I factor is not a member of the copia-like or fold-back classes of transposable elements and has no sequence homology with the P factor that controls P-M dysgenesis. All strains of D. melanogaster contain I-factor sequences. Those present in reactive strains must represent inactive I elements. I elements have a remarkably similar sequence organization in all reactive strains and are located in peri-centromeric regions. Inducer strains appear to contain both I elements, located in pericentromeric regions, and 10-15 copies of the complete I factor at sites on the chromosome arms.