Microscopic birefringence imaging by the local-sampling phase shifting technique
Microscopic birefringence imaging by the local-sampling phase shifting technique
复制标题
通过局部采样相移技术进行显微双折射成像
DOI：
10.1117/12.547768
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
N. Umeda
中科院分区：
文献类型：
--
作者：
Mizue Ebisawa;Y. Otani;N. Umeda
提出了一种生物样品的显微双折射成像方法。该系统由超辐射发光二极管（SLD）、偏振片、四分之一波片和相位延迟器组成。提供该仪器以映射和可视化生物样品中的光学各向异性。采用局部采样相移技术，实现了高分辨率的相位延迟解析算法。并以Bereck补偿器为例进行了精度检验.作为演示，给出了外加电压和温度变化情况下明胶取向的双折射率分布，如相位差和方位角方向。可以观察生物样品的分子取向。
A microscopic birefringence imaging of bio-sample is proposed. This system consists of a super luminescent diode (SLD), polarizers, a quarter-wave plate and a phase retarder. The instrument is provided to map and visualize an optical anisotropy in bio-sample. A local-sampling phase shifting technique is employed for analytic algorithm with high resolution of retardance. A Bereck compensator is used a sample for checking its accuracy. Birefringence distributions of gelatin orientation such as retardance and azimuthal direction are shown in case of applied voltage and changing temperature as its demonstration. It is possible to observe molecular orientation of bio-sample.