Postnatal rat sympathetic neurons in culture. II. Synaptic transmission by postnatal neurons.
Postnatal rat sympathetic neurons in culture. II. Synaptic transmission by postnatal neurons.
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培养中的出生后大鼠交感神经元。
DOI：
10.1152/jn.1979.42.5.1426
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发表时间：
1979
影响因子：
2.5
通讯作者：
H. Burton
中科院分区：
文献类型：
--
作者：
E. Wakshull;M. I. Johnson;H. Burton
1.在前一篇论文中，我们发现出生后的大鼠上级颈神经节神经元（SCGN）可以在分离的细胞培养中生长，并形成功能性的突触连接。在这份报告中，突触传递的出生后SCGN的详细。2. SCGN之间的突触相互作用被烟碱胆碱能拮抗剂六甲铵（C-6）阻断，表明乙酰胆碱是这些神经元使用的递质物质。即使是从12.5周龄的动物身上提取的神经元也是如此。3.在少数情况下，β-肾上腺素能阻断剂普萘洛尔被发现可以阻断突触电位，这表明儿茶酚胺可能参与了传递过程。这种参与的可能机制进行了讨论。4.取自10周龄大鼠的SCGN能够与共培养的骨骼肌细胞形成功能性突触接触。这些相互作用对低外部Ca 2+和1- 2 μ M d-筒箭毒碱（d-TC）敏感。5.它的结论是，即使成人SCGN保留一定量的神经递质“可塑性”，在适当的培养条件下生长。从神经元-神经元和SCGN-骨骼肌相互作用的数据，这表明，突触前递质与突触后受体的匹配是形成功能性神经-靶相互作用的充分条件。
1. It was shown in the preceding paper that postnatally derived rat superior cervical ganglion neurons (SCGN) will grow in dissociated cell culture and form functional synaptic connections with each other. In this report, synaptic transmission by the postnatal SCGN is detailed. 2. Synaptic interactions between SCGN were blocked by the nicotinic cholinergic antagonist hexamathonium (C-6), indicating that acetylcholine was the transmitter substance used by these neurons. This was found to be the case even for neurons taken from 12.5-wk-old animals. 3. In a few cases, the beta-adrenergic blocking agent, propranolol, was found to block synaptic potentials, suggesting that a catecholamine might be involved in the transmission process. The possible mechanisms of this involvement are discussed. 4. SCGN taken from up to 10-wk-old rats were able to form functional synaptic contacts with cocultured skeletal muscle cells. These interactions were sensitive to low external Ca2+ and to 1--2 microM d-tubocurarine (d-TC). 5. It is concluded that even adult SCGN retain a certain amount of neurotransmitter "plasticity" when grown under appropriate culture conditions. From the data on the neuron-neuron and SCGN-skeletal muscle interactions, it is suggested that a matching of presynaptic transmitter with postsynaptic receptor is a sufficient condition for the formation of functional nerve-target interactions.