Synthesis and hybridization properties of iridium(III) polypyridyl complex-conjugated oligonucleotide
Synthesis and hybridization properties of iridium(III) polypyridyl complex-conjugated oligonucleotide
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DOI：
10.1080/15257770.2019.1690150
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发表时间：
2020-01
期刊：
影响因子：
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通讯作者：
Yuta Ito;K. Mizuno;Kohei Domoto;Y. Hari
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文献类型：
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作者：
Yuta Ito;K. Mizuno;Kohei Domoto;Y. Hari
摘要 合成了Ir(III)聚吡啶复合物缀合的14聚体寡核苷酸(IrIII-DNA)，并通过紫外熔解实验评估了其与单链DNA(ssDNA)和双链DNA(dsDNA)的杂交特性。 IrIII-DNA 与 14、20 和 26 聚体 ssDNA 的双链体稳定性高于未缀合的寡核苷酸。 IrIII-DNA 与 14-mer dsDNA 的三链体也得到了稳定。然而，IrIII-DNA 与 20 聚体和 26 聚体 dsDNA 的三链体（侧翼为 14 聚体 dsDNA 靶标两端的 3 个和 6 个碱基对）不稳定。这可能是由于 Ir(III) 复合物与突出的 3 聚体和 6 聚体 dsDNA 部分之间的空间排斥所致，与 ssDNA 相比，这些结构是刚性的。
Abstract An Ir(III) polypyridyl complex-conjugated 14-mer oligonucleotide (IrIII-DNA) was synthesized and its hybridization properties with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) were evaluated by UV-melting experiments. The stabilities of the duplexes of IrIII-DNA with 14-, 20-, and 26-mer ssDNAs were higher than those of the unconjugated oligonucleotides. The triplex of IrIII-DNA with 14-mer dsDNA was also stabilized. However, the triplexes of IrIII-DNA with 20- and 26-mer dsDNAs, flanked by 3 and 6 base pairs at the both ends of 14-mer dsDNA target, were destabilized. This is presumably because of steric repulsion between the Ir(III) complex and the protruding 3- and 6-mer dsDNA moieties which are inflexible compared to ssDNA.