CERVICAL MUCUS NEUTROPHILS FUNCTIONAL STATUS INTERACTION WITH THE SPECIES COMPOSITION OF VAGINAL LACTOBACILLI IN PREGNANT WOMEN
CERVICAL MUCUS NEUTROPHILS FUNCTIONAL STATUS INTERACTION WITH THE SPECIES COMPOSITION OF VAGINAL LACTOBACILLI IN PREGNANT WOMEN
复制标题
孕妇宫颈粘液中性粒细胞功能状态与阴道乳酸杆菌种类组成的相互作用
DOI：
10.36233/0372-9311-2018-4-51-56
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
I. Dolgushin
中科院分区：
文献类型：
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作者：
Y. Shishkova;V. Dolgushina;E. Grafová;S. A. Zavyalova;I. Kurnosenko;N. Evstigneeva;K. Gromakova;O. Kolesnikov;A. V. Chukichev;I. Dolgushin
瞄准本研究的目的是评估妊娠早期妇女宫颈粘液中性粒细胞功能状态对优势菌株的依赖性。材料和方法。本文对40株从孕妇生殖道分离到的大肠埃希菌进行了鉴定。我们使用质谱分析来鉴定阴道乳酸杆菌并评估生物膜形成。此外，我们还研究了宫颈粘液中性粒细胞的活力，溶酶体活性，自发和诱导NBT试验（Nitroblue Tetrazolium试验），吸收乳胶颗粒的能力。根据是否存在阴道或宫颈感染性疾病，将分离菌株分为3组。结果共鉴定出5种乳酸杆菌：L. jensenii湖mspatus，L.阿托菲勒斯湖加塞里湖delbrueckii在40个确定的菌株中。我们确定了不同的吞噬强度取决于优势菌株在normocenosis。与L.与L.嗜酸乳杆菌（L.acidophilus）和詹氏乳杆菌（L.jensenii）。在生殖器感染的情况下能够密集产生生物基质的乳酸杆菌。从而阻断抗原潜能，导致嗜中性粒细胞的NBT还原能力缺失，降低孕妇生殖道粘膜的抗菌保护作用。结论妊娠早期妇女中性粒细胞功能状态的指标取决于优势乳杆菌菌株。
Aim. Of the study is to assess cervical mucus neutrophils functional status depend on dominant lactobacillus strain in I trimester of pregnancy women. Material and methods. We definded 40 lactobacillus strains obtained from the genital tract of the pregnant women. We used mass spectrometry analysis to identify vaginal lactobacilli and evaluated biofilm formation. Also, we studied cervical mucus neutrophils viability, lysosomal activity, spontaneous and induced NBT test (Nitroblue Tetrazolium test), ability to absorb the latex particles. Isolated strains were divided into 3 groups depending on presence or absence of vaginal or cervical channel infectious diseases. Results. We identified 5 lactobacilli types: L. jensenii, L. mspatus, L. аcidophilus, L. gasseri, L. delbrueckii among 40 definded strains. We determined different intensity of phagocytosis depending on dominant strain in normocenosis. With the L. crispatus prevalence demonstrated the least intensity of phagocytosis compared with L. acidophilus and L.jensenii. Lactobacilli able to intensively produce biomatrix in condition of genital infection. Thus blocking antigenic potential and result in absence of NBT-reducing power of neutrophilic granulocytes and reduction antimicrobial protection of pregnant women genital tract mucosa. Conclusion. The indicators of neutrophils functional status depend on dominant lactobacilli strain in I trimester of pregnancy women.