Assessing the prevalence of Salmonella enterica in poultry hatcheries by using hatched eggshell membranes
Assessing the prevalence of Salmonella enterica in poultry hatcheries by using hatched eggshell membranes
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DOI：
10.1093/ps/86.8.1651
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发表时间：
2007-08-01
期刊：
影响因子：
4.4
通讯作者：
Yu, C.-Y.
中科院分区：
文献类型：
--
作者：
Chao, M.-R.;Hsien, C.-H.;Yu, C.-Y.
肠道沙门氏菌引起许多重要的家禽疾病，也是人类的主要病原体。大多数感染沙门氏菌的家禽成为带菌者，感染也可能是致命的，这取决于特定的血清型和感染时鸟的年龄。幼鸟更容易感染沙门氏菌，因此孵化场对鸟类进行监测至关重要。我们开发了一种方法，使用孵化蛋壳膜（HEM），以评估沙门氏菌的家禽孵化柜中的污染，并评估沙门氏菌在鹅孵化场和饲养场的流行。3种采样方法对孵化柜中沙门氏菌检出率的比较表明，HEM中沙门氏菌检出率最高，与绒毛样品和孵化柜拭子样品的检出率差异显著（P < 0.05）。HEM的分析也被用来评估沙门氏菌污染的鹅，鸡，鸭孵化场。沙门氏菌阳性率以鸡场最低，其次为鹅场和鸭场（P < 0.05）。3个孵化场共检出6个血清型的沙门氏菌：A、B、C1、C2、D和E。这些血清群的分布不同的孵化场。沙门氏菌血清群C1是在鹅中发现的主要血清群，而血清群B在鸡和鸭中发现。然而，鼠伤寒沙门氏菌是占主导地位的1鹅孵化场，也在鹅从这个孵化场已转移到一个农场。药敏结果显示，从腹泻鹅群中分离的鼠伤寒沙门氏菌对强力霉素、粘菌素、磺胺甲恶唑-甲氧苄氨嘧啶和头孢噻吩的耐药性显著高于孵化场分离的菌株（P <0. 05）。因此，HEM作为检测目标可用于监测孵化柜中的沙门氏菌污染，也可用于评估家禽孵化场和饲养场中沙门氏菌的流行情况。
Salmonella enterica causes a number of significant poultry diseases and is also a major pathogen in humans. Most poultry infected by Salmonella become carriers, infection may also be fatal, depending on the particular serovar and the age of the bird at infection. Younger birds are more susceptible to infection by Salmonella, so it is critical that hatcheries monitor birds. We developed a method to use hatched eggshell membranes (HEM) to assess contamination by Salmonella in poultry hatching cabinets and to evaluate the prevalence of Salmonella in a goose hatchery and rearing farm. Comparison of the Salmonella isolation rate in hatching cabinets using 3 sampling methods showed that the highest Salmonella contamination was detected in HEM, and that these results differed significantly from those obtained from fluff samples and cabinet swab samples (P < 0.05). Analysis of HEM was also used to evaluate Salmonella contamination in goose, chicken, and duck hatcheries. The lowest Salmonella-positive rate was found for the chicken hatchery, followed by the goose and the duck hatcheries (P < 0.05). Six serogroups of Salmonella were detected in the 3 hatcheries: A, B, C1, C2, D, and E. The distribution of these serogroups differed among the hatcheries. Salmonella serogroup C1 was the major serogroup found in geese, compared with serogroup B in chickens and ducks. However, Salmonella Typhimurium was dominant in 1 goose hatchery and also in geese from this hatchery that had been transferred to a farm. Antibiotic susceptibility analysis showed that Salmonella Typhimurium strains isolated from the farm geese with diarrhea showed significantly higher resistance to doxycycline, colistin, sulfamethoxazole-trimethoprin, and cephalothin than those isolated from the hatchery (P < 0.05). Therefore, HEM as a detection target can be used to monitor Salmonella contamination in hatching cabinets and also be used to assess Salmonella prevalence in poultry hatcheries and rearing farms.