A type I and type II microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) with presumptive coverage of all chromosome arms.
A type I and type II microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) with presumptive coverage of all chromosome arms.
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DOI：
10.1186/1471-2164-7-302
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发表时间：
2006-11-30
期刊：
影响因子：
4.4
通讯作者：
Quillet, Edwige
中科院分区：
文献类型：
--
作者：
Guyomard, Rene;Mauger, Stephane;Tabet-Canale, Kamila;Martineau, Sylvain;Genet, Carine;Krieg, Francine;Quillet, Edwige
大型基因组资源的开发已成为阐明基因组大规模进化以及复杂性状分子基础的先决条件。连锁图谱代表了对这类资源整合和利用的初级阶段，也是数量性状分子分析的主要框架。先前发表的连锁图谱已经勾勒出虹鳟减数分裂的主要特征，并且最近利用荧光原位杂交技术已经确定了连锁群和染色体臂之间的对应关系。这些图谱所覆盖的染色体臂数量仍然未知。
我们报道了一个更新的连锁图谱，它基于对两个双单倍体雌核发育系中900多个微卫星标记的分离分析。这些标记分离成31个连锁群，覆盖的总图谱长度约为2750厘摩。利用减数分裂系对所有连锁群的着丝粒进行了定位。对于31个连锁群中的每一个，从着丝粒定位推断出的中着丝粒或近端着丝粒结构与最近通过荧光原位杂交结果发现的结构相同。因此，假定当前的图谱覆盖了构成虹鳟核型的52个染色体臂。我们的数据证实了该物种中存在高水平的干扰。在11个连锁群中鉴定出了同源区域，反映了鲑科基因组的四倍体性质。数据支持了这样的假设：重复群之间基因顺序是保守的，并且每个群位于单个染色体臂上。总体而言，在基因同线性和顺序方面，与已发表的虹鳟连锁图谱高度一致。
这个新图谱可能覆盖了整套染色体臂，应该为整合现有的或即将出现的虹鳟连锁图谱和其他基因组资源提供一个有用的框架。由于数据库中有大量包含微卫星序列的表达序列标签（EST），构建定位已知基因的高密度连锁图谱变得可行。这将促进比较图谱绘制，并最终在数量性状位点（QTL）研究中鉴定候选基因。
The development of large genomic resources has become a prerequisite to elucidate the wide-scale evolution of genomes and the molecular basis of complex traits. Linkage maps represent a first level of integration and utilization of such resources and the primary framework for molecular analyses of quantitative traits. Previously published linkage maps have already outlined the main peculiarities of the rainbow trout meiosis and a correspondance between linkage groups and chromosome arms has been recently established using fluorescent in situ hybridization. The number of chromosome arms which were covered by these maps remained unknown. We report an updated linkage map based on segregation analysis of more than nine hundred microsatellite markers in two doubled haploid gynogenetic lines. These markers segregated into 31 linkage groups spanning an approximate total map length of 2750 cM. Centromeres were mapped for all the linkage groups using meiogenetic lines. For each of the 31 linkage groups, the meta or acrocentric structure infered from centromere mapping was identical with those recently found with fluorescent in situ hybridization results. The present map is therefore assumed to cover the 52 chromosome arms which constitute the rainbow trout karyotype. Our data confirm the occurrence of a high interference level in this species. Homeologous regions were identified in eleven linkage groups, reflecting the tetraploid nature of the salmonid genome. The data supported the assumption that gene orders are conserved between duplicated groups and that each group is located on a single chromosome arm. Overall, a high congruence with already published rainbow trout linkage maps was found for both gene syntenies and orders. This new map is likely to cover the whole set of chromosome arms and should provide a useful framework to integrate existing or forthcoming rainbow trout linkage maps and other genomic resources. Since very large numbers of EST containing microsatellite sequences are available in databases, it becomes feasible to construct high-density linkage maps localizing known genes. This will facilitate comparative mapping and, eventually, identification of candidate genes in QTL studies.