Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds
Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds
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DOI：
10.1016/s0142-9612(00)00280-5
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发表时间：
2001-06-01
期刊：
影响因子：
14
通讯作者：
Mikos, AG
中科院分区：
文献类型：
--
作者：
Goldstein, AS;Juarez, TM;Mikos, AG
在体外三维骨传导支架中培养成骨细胞是一种很有前途的制备骨诱导材料用于修复骨缺损的方法，然而，在足够大的支架中培养细胞以桥接临界尺寸的缺损对组织工程师来说是一个挑战。扩散可能不足以将营养物供应到大支架中，因此细胞可能在静态培养条件下优先在周边生长。考虑了对流培养基的三种替代培养方案：旋转瓶、旋转容器和灌注流系统。将聚（DF-乳酸-共-乙醇酸）（PLGA）泡沫盘（直径12.7 mm，厚6.0 mm，78.8%多孔）与成骨骨髓基质细胞接种，并在地塞米松和L-抗坏血酸存在下培养7天和14天。发现每个泡沫的细胞数量与所有培养方案相似，表明对流不能增强细胞生长，但组织学分析表明旋转容器和Row系统在整个泡沫中产生更均匀的细胞分布。碱性磷酸酶（ALP）的活性，每个细胞的培养在流动系统和转瓶7天后，而骨钙素（OC）的活性没有差异，每个细胞的培养方法之间观察培养14天后。基于在整个培养的泡沫中较高的ALP活性和较好的细胞均匀性，流动系统似乎是上级培养方法，尽管同样重要的事实是，在所有试验中，任何替代培养技术的表现都不低于静态对照。因此，本研究表明，利用流体行的培养技术，特别是流动灌注系统，与静态培养中保持的那些相比，改善了接种细胞的性质，（C）2001 Elsevier Science Ltd.保留所有权利。
Culture of seeded osteoblastic cells in three-dimensional osteoconductive scaffolds in vitro is a promising approach to produce an osteoinductive material for repair of bone defects, However, culture of cells in scaffolds sufficiently large to bridge critical-sized defects is a challenge for tissue engineers. Diffusion may not be sufficient to supply nutrients into large scaffolds and consequently cells may grow preferentially at the periphery under static culture conditions. Three alternative culturing schemes that convect media were considered: a spinner flask, a rotary vessel, and a perfusion flow system. Poly(DF-lactic-co-glycolic acid) (PLGA) foam discs (12.7 mm diameter, 6.0 mm thick, 78.8% porous) were seeded with osteoblastic marrow stromal cells and cultured in the presence of dexamethasone and L-ascorbic acid for 7 and 14 days. Cell numbers per foam were found to be similar with all culturing schemes indicating that cell growth could not be enhanced by convection, but histological analysis indicated that the rotary vessel and Row system produced a more uniform distribution of cells throughout the Foams. Alkaline phosphatase (ALP) activity per cell was higher with culture in the flow system and spinner flask after 7 days, while no differences in osteocalcin (OC) activity per cell were observed among culturing methods after 14 days in culture. Based on the higher ALP activity and better cell uniformity throughout the cultured foams, the flow system appears to be the superior culturing method, although equally important is the fact that in none of the tests did any of the alternative culturing techniques underperform the static controls. Thus, this study demonstrates that culturing techniques that utilize fluid Row, and in particular the flow perfusion system, improve the properties of the seeded cells over those maintained in static culture, (C) 2001 Elsevier Science Ltd. All rights Reserved.