RIBONUCLEIC ACID OF AXONS AND MYELIN SHEATHS FROM MAUTHNER NEURONS
RIBONUCLEIC ACID OF AXONS AND MYELIN SHEATHS FROM MAUTHNER NEURONS
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DOI：
10.1016/0926-6550(62)90080-4
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发表时间：
1962-01-01
期刊：
影响因子：
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通讯作者：
EDSTROM, A
中科院分区：
文献类型：
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作者：
EDSTROM, JE;EICHNER, D;EDSTROM, A
用显微操作方法从固定的金鱼脊髓中分离出配对的巨型毛特纳神经元轴突和髓鞘。用核糖核酸酶提取分离的细胞，并用超微化学方法研究提取物的RNA含量和组成。得到了以下结果：毛特纳神经元的轴突和髓鞘中存在RNA。每单位纤维长度的RNA量在轴突和髓鞘中是相同数量级的。与神经细胞体相比，轴突和髓鞘每固定体积的RNA浓度分别低10倍和40倍。据估计，轴突的RNA总量是细胞体的4倍。与神经细胞体的RNA相比，轴突和髓鞘的RNA在碱基组成上有很大的差异，与核糖体RNA有明显的不同。
Axons and myelin sheaths from the paired giant Mauthner neuron were isolated from fixed spinal cords of the goldfish by means of micromanipulation. Isolated cells were extracted with ribonuclease and the extracts were investigated for RNA content and composition using ultramicrochemical procedures. The following results were obtained. RNA is present in axons and myelin sheaths of Mauthner neurons. The amount of RNA per unit fibre-length is of the same order for axon and myelin sheath. The concentration of RNA for axons and myelin sheaths per fixed volume compared to that of the nerve cell body is 10 and 40 times lower, respectively. The total amount of RNA in the axon is estimated to be 4 times greater than that of the cell body. The RNA of axons and myelin sheaths shows great variations in base composition and differs distinctly from ribo-somal RNA, in contrast to the RNA of nerve cell bodies.