Ascorbate biosynthesis in Arabidopsis cell suspension culture
Ascorbate biosynthesis in Arabidopsis cell suspension culture
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DOI：
10.1104/pp.121.2.535
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发表时间：
1999-10-01
期刊：
影响因子：
7.4
通讯作者：
Van Montagu, MC
中科院分区：
文献类型：
--
作者：
Davey, MW;Gilot, C;Van Montagu, MC
以拟南芥（L.）嘿通过定量与一系列潜在的生物合成前体、类似物和抑制剂一起孵育对细胞内还原和氧化形式的L-AA水平的影响来研究细胞悬浮培养。我们的研究结果支持最近发表的从L-半乳糖（G. L.惠勒，文学硕士琼斯，N。Smirnoff [1998] Nature 393：365-369），但提出拟南芥细胞悬浮培养物同时含有导致L-AA的两种其它途径。这些替代路线可能的生理意义进行了讨论。
The biosynthesis of L-ascorbic acid (L-AA) in an Arabidopsis (L.) Heynh. cell suspension culture was studied by quantifying the effects of incubation with a range of potential biosynthetic precursors, analogs, and inhibitors on the intracellular levels of reduced and oxidized forms of L-AA. Our results support the recently published biosynthetic pathway of L-AA from L-galactose (G.L. Wheeler, M.A. Jones, N. Smirnoff [1998] Nature 393: 365-369), but suggest that Arabidopsis cell suspension culture simultaneously contains two other routes leading to L-AA. The possible physiological significance of these alternate routes is discussed.