The influence of ligand organization on the rate of uptake of gold nanoparticles by colorectal cancer cells
The influence of ligand organization on the rate of uptake of gold nanoparticles by colorectal cancer cells
复制标题
DOI：
10.1016/j.biomaterials.2011.09.018
复制
发表时间：
2011-12-01
期刊：
影响因子：
14
通讯作者：
Bayford, Richard
中科院分区：
文献类型：
--
作者：
Lund, Torben;Callaghan, Martina F.;Bayford, Richard
我们探索了不同亲水性的单、双配体金纳米粒子在体外对结直肠癌细胞的摄取，发现摄取的速度取决于配体在粒子表面的结构组织，而不是它们的电荷或化学性质。含有50%PEGNH2/50%葡萄糖的金纳米颗粒的吸收速度是只含有PEGNH2或葡萄糖的纳米颗粒的18倍。到目前为止，包被谷胱甘肽的金颗粒是最有效的内化；然而，葡萄糖-谷胱甘肽双配体纳米颗粒的吸收速度降低了30倍。此外，我们还发现，细胞密度和生长介质中葡萄糖的浓度对这一速率有影响。这些颗粒不是通过传统的受体介导的机制被内化，而是通过金纳米颗粒上的配体在脂质双层中预先存在的孔或开口，通过跨细胞膜的能量独立扩散被细胞吸收。(C)2011爱思唯尔有限公司。保留所有权利。
We have explored the uptake of different hydrophilic mono- and dual-ligand gold nanoparticles in colorectal cancer cells in vitro and find that the rate of uptake is dependent on the structural organization of the ligands on the surface of the particles rather than their charge or chemical properties. Gold nanoparticles with 50%PEG-NH2/50% glucose are taken up eighteen fold faster than nanoparticles carrying only PEG-NH2 or glucose. Glutathione-coated gold particles are by far the most efficiently internalized; however, glucose-glutathione dual-ligand nanoparticles are taken up at a thirty fold reduced rate. We found furthermore that the rates are influenced by the cell density and concentration of glucose in the growth medium. Rather than being internalized through a conventional receptor-mediated mechanism the particles appear to be taken up by the cells via an energy-independent diffusion across the cell membrane through pre-existing pores or openings in the lipid bi-layer created by ligands on the gold nanoparticles. (C) 2011 Elsevier Ltd. All rights reserved.