Genome-wide identification and characterization of basic helix-loop-helix transcription factors in Spodoptera litura upon pathogen infection
Genome-wide identification and characterization of basic helix-loop-helix transcription factors in Spodoptera litura upon pathogen infection
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斜纹夜蛾病原体感染后基本螺旋-环-螺旋转录因子的全基因组鉴定和表征
DOI：
10.1111/1744-7917.12979
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发表时间：
2021-12-06
期刊：
影响因子：
4
通讯作者：
Lu，Yuzhen
中科院分区：
文献类型：
--
作者：
Zhang，Jie;Zhu，Mengyao;Lu，Yuzhen
碱性螺旋-环-螺旋(BHLH)转录因子在真核生物的代谢和发育过程中发挥着重要作用，bHLH蛋白也参与免疫反应，特别是在植物中。然而，它们在昆虫对昆虫病原侵染中的作用尚不清楚。在本研究中，在多食性害虫斜纹夜蛾中发现了41个科的54bHLH基因，其中在B组中发现了一个新的bHLH基因，该基因是鳞翅目特有的，因此命名为Lep。BHLHINS的bHLH域的保守氨基酸、结构构型和染色体分布。并对其进行了分析。还更新了小菜蛾和意大利蜜蜂的bHLH基因，并对5种全变态昆虫的bHLH成员进行了全基因组比较和系统发育分析。表达轮廓为OFS。斜纹夜蛾不同发育阶段3种组织中的bHLH基因及其对S的反应。调查了斜纹夜蛾核型多角体病毒(SpltNPV)、里氏无核线虫(Nomuraea Rileyi)和苏云金芽孢杆菌(Bt)的感染情况。在病原体感染过程中，B组有较多的SLbHLHsin表达和差异表达，而SLbHLHh在中肠OFS中的表达有下调趋势。B之后的斜纹。苏云金耐药治疗。我们的研究提供了bHLH家族成员INS的概述。以及它们对用于害虫生物防治的不同病原菌的反应。这些发现可能有助于揭示宿主与病原体相互作用的机制。
Basic helix‐loop‐helix (bHLH) transcription factors play an important role in a wide range of metabolic and developmental processes in eukaryotes, and bHLH proteins also participate in immune responses, especially in plants. However, their roles in insects upon entomopathogen infection are unknown. In this study, 54bHLHgenes in 41 families were identified in a polyphagous pest,Spodoptera litura, including a newbHLHgene in group B, which is specifically present in Lepidoptera and was thus namedLep. The conserved amino acids in the bHLH domain, structural architecture, and chromosomal distribution ofbHLHgenes inS. liturawere analyzed. ThebHLHgenes inPlutella xylostellaandApis melliferawere also updated, and genome‐wide comparison and phylogenetic analysis of bHLH members in 5 holometabolous insects were performed. The expression profiles ofS. litura bHLH(SlbHLH) genes in 3 tissues at different developmental stages and their responses toS. lituranucleopolyhedrovirus (SpltNPV),Nomuraea rileyi(Nr), andBacillus thuringiensis(Bt) infection were investigated. MoreSlbHLHsin group B were expressed and differentially expressed during pathogen infections, andSlbHLHstended to be downregulated in the midgut ofS. lituralarvae afterB. thuringiensistreatment. Our study provides an overview of bHLH family members inS. lituraand their responses to different pathogens used for pest biocontrol. These findings on bHLH members may contribute to uncovering the mechanism of host–pathogen interaction.