DRhoGEF2 encodes a member of the Db1 family of oncogenes and controls cell shape changes during gastrulation in Drosophila
DRhoGEF2 encodes a member of the Db1 family of oncogenes and controls cell shape changes during gastrulation in Drosophila
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DOI：
10.1101/gad.12.2.274
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发表时间：
1998-01-15
影响因子：
10.5
通讯作者：
Perrimon, N
中科院分区：
文献类型：
--
作者：
Häcker, U;Perrimon, N
我们已经确定了一个基因，DRhoGEF2，它编码一个假定的鸟嘌呤核苷酸交换因子属于Dbl家族的癌基因。DRhoGEF2功能对于原肠胚形成期间细胞形状变化的协调至关重要。在没有母体DRhoGEF2基因活性的情况下，中胚层和内胚层原基不能内陷。在DRhoGEF 2突变体中观察到的表型比与先前鉴定的两个原肠胚形成基因（折叠原肠胚形成和手风琴）中的突变相关的缺陷更严重，这表明DRhoGEF 2在独立于这些的信号通路中起作用。在原肠胚形成过程中显性负性DRhoA导致DRhoGEF2突变体的表型，表明涉及DRhoGEF2和小GTfdDRhoA的信号级联反应负责调节果蝇早期形态发生过程中的细胞形状变化。
We have identified a gene, DRhoGEF2, which encodes a putative guanine nucleotide exchange factor belonging to the Dbl family of oncogenes. DRhoGEF2 function is essential for the coordination of cell shape changes during gastrulation. In the absence of maternal DRhoGEF2 gene activity, mesodermal and endodermal primordia fail to invaginate. The phenotype seen in DRhoGEF2 mutants is more severe than the defects associated with mutations in two previously identified gastrulation genes, folded gastrulation and concertina, suggesting that DRhoGEF2 acts in a signaling pathway independent of these. dominant-negative DRhoA during gastrulation results in phenocopies of the DRhoGEF2 mutant, suggesting that a signaling cascade involving DRhoGEF2 and the small GTPase DRhoA is responsible for the regulation of cell shape changes during early Drosophila morphogenesis.