Physicochemical analysis of initial adhesion and biofilm formation of Methanosarcina barkeri on polymer support material.
Physicochemical analysis of initial adhesion and biofilm formation of Methanosarcina barkeri on polymer support material.
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DOI：
10.1016/j.colsurfb.2016.03.042
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发表时间：
2016-07
期刊：
影响因子：
--
通讯作者：
Vi Nguyen;E. Karunakaran;G. Collins;C. Biggs
中科院分区：
文献类型：
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作者：
Vi Nguyen;E. Karunakaran;G. Collins;C. Biggs
在厌氧消化池内保留选择性生物膜可减少启动时间，提高处理高浓度生活污水的效率。本研究的目的是考察6种常见聚合物载体材料的表面特性对模拟产甲烷菌--巴氏甲烷链霉菌初始粘附性的影响，并评价这些载体材料作为选择性生物膜载体的潜力。初始黏附试验和扩展DLVO(XDLVO)模型的结果相互关联，PVC(12%表面覆盖率/mm2)，PTFE(6%表面覆盖率/mm2)，PP(6%表面覆盖率/mm2)，被证明是更好的初始黏附和后续M生物膜形成的支撑材料。这三种载体材料的实验结果表明，材料的类型对粘附性的影响很大。Barkeri(p<；t；0.0001)，xDLVO模型能够解释在这些环境条件下的结果。因此，我们发现DLVO的物理化学力对FM的初始粘附力有影响。巴克里。扫描电子显微镜显示细胞外聚合物(EPS)的产生。树皮可以促进生物膜的进一步发展。这项研究强调了使用xDLVO模型快速确定适合FM选择性黏附的材料的潜力。Barkeri，这在厌氧消化的启动阶段和长期阶段都是有益的。
The retention of selective biofilms ofMethanosarcinaspecies within anaerobic digesters could reduce start-up times and enhance the efficiency of the process in treating high-strength domestic sewage. The objective of the study was to examine the effect of the surface characteristics of six common polymer support materials on the initial adhesion of the model methanogen,Methanosarcina barkeri, and to assess the potential of these support materials as selective biofilm carriers. Results from both the initial adhesion tests and extended DLVO (xDLVO) model correlated with each other, with PVC (12% surface coverage/mm2), PTFE (6% surface coverage/mm2), and PP (6% surface coverage/mm2), shown to be the better performing support materials for initial adhesion, as well as subsequent biofilm formation byM. barkeriafter 72 h. Experimental results of these three support materials showed that the type of material strongly influenced the extent of adhesion fromM. barkeri(p < 0.0001), and the xDLVO model was able to explain the results in these environmental conditions. Therefore, DLVO physicochemical forces were found to be influential on the initial adhesion ofM. barkeri. Scanning electron microscopy suggested that production of extracellular polymeric substances (EPS) fromM. barkericould facilitate further biofilm development. This study highlights the potential of using the xDLVO model to rapidly identify suitable materials for the selective adhesion ofM. barkeri, which could be beneficial in both the start-up and long-term phases of anaerobic digestion.