Lysosomal enzymes of Paramecium caudatum and Paramecium tetraurelia.
Lysosomal enzymes of Paramecium caudatum and Paramecium tetraurelia.
复制标题
尾草履虫和四尿草履虫的溶酶体酶。
DOI：
10.1016/0014-4827(82)90329-9
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发表时间：
1982
影响因子：
3.7
通讯作者：
Paeste,RM
中科院分区：
文献类型：
--
作者：
Fok,AK;Paeste,RM
在 Soldo 等人粗略确定的培养基中生长的尾草履虫和四尿草履虫中发现了 16 种水解酶活性。 [15]。细胞蛋白质和大多数酶活性之间的比率。尾部和 P.四尿利亚为 3-4。酸性磷酸酶（使用 β-甘油磷酸盐和对硝基苯磷酸盐作为底物）、核糖核酸酶、β-葡萄糖苷酶和组织蛋白酶 B 被证明具有酸性 pH 最适值，并且位于通过差速离心获得的溶酶体级分中。当这些纤毛虫经历培养周期时，观察到水解酶活性的可预测变化。两个物种的蛋白质含量和酶活性在对数早期达到最大值，并在第 5 天和第 6 天（P 稳定期的开始）下降到较低水平。四尿利亚和 P.尾部，分别。在稳定期，组织蛋白酶 C、β-葡萄糖苷酶和 DNase inP。 caudatum 保持相对不变，而 β-甘油磷酸酶 (βGPase) 和 RNase 进一步下降，但 P.四尿素菌所有四种水解酶活性均显示中度降低。在对数期观察到水解酶比活性 (SA) 变化的类似趋势，但在稳定期 SA 的变化有所不同，因为 SA 反映了活性/细胞和蛋白质/细胞之间的相对变化。 P 中的 DNA 酶、组织蛋白酶 C 和 β-葡萄糖苷酶。 caudatum，以及 βGPase、组织蛋白酶 C 和 RNase inP。 tetraurelia显示SA增加，而P中βGPase和RNase的SA增加。 P 中 β-葡萄糖苷酶的尾部。 tetraurelia 拒绝了。这些结果表明先前报道的在 P 的稳定期观察到的溶酶体增殖。 caudatum[11] 与溶酶体酶活性的任何增加无关，而是与溶酶体酶活性降低相关。
Sixteen hydrolase activities were found to be present inParamecium caudatumandParamecium tetraureliagrowing in the crudely defined medium of Soldo et al. [15]. The ratios of cellular protein and most enzyme activities betweenP. caudatumandP. tetraureliawere 3–4. Acid phosphatase (using both β-glycerophosphate andp-nitrophenylphosphate as substrates), ribonuclease, β-glucosidase and cathepsin B were shown to have acidic pH optima and to be localized in the lysosomal fraction obtained by differential centrifugation. Predictable changes in hydrolase activities were observed as these ciliates passed through the culture cycle. Protein content and enzyme activities in both species reached a maximum during early log phase and declined to lower levels on days 5 and 6, the beginning of stationary phase forP. tetraureliaandP. caudatum, respectively. During stationary phase cathepsin C, β-glucosidase and DNase inP. caudatumremained relatively unchanged, whereas β-glycerophosphatase (βGPase) and RNase declined further, but inP. tetraureliaall four hydrolase activities showed moderate decreases. A similar trend in specific activity (SA) changes of the hydrolases was observed during log phase, but the changes in SA during stationary phase varied as SA reflects the relative changes between activity/ cell and protein/cell. DNase, cathepsin C and β-glucosidase inP. caudatum, and βGPase, cathepsin C and RNase inP. tetraureliashowed increases in SA, whereas the SA of βGPase and RNase inP. caudatumand of β-glucosidase inP. tetraureliadeclined. These results indicate that the previously reported proliferation of lysosomes observed during the stationary phase ofP. caudatum[11] is not correlated with any increase but a decrease in lysosomal enzyme activities.