Complementary seminovaginal microbiome in couples
Complementary seminovaginal microbiome in couples
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DOI：
10.1016/j.resmic.2015.03.009
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发表时间：
2015-06-01
影响因子：
2.6
通讯作者：
Truu, Jaak
中科院分区：
文献类型：
--
作者：
Maendar, Reet;Punab, Margus;Truu, Jaak
生殖道微生物群与生殖健康密切相关。虽然已经对阴道微生物群进行了许多研究，但目前对男性微生物群的了解很少，而且对夫妇进行平行研究的情况也非常罕见。在这项工作中，我们旨在比较夫妇的精液和阴道微生物群，并评估性交对阴道微生物的影响。研究包括23对夫妇。用Illumina HiSeq2000 16S rRNA基因V6区序列分析了精液和阴道液(性交前后)的微生物群落。精液群落的多样性明显高于阴道，但总细菌浓度低于阴道。在伴侣患有严重白细胞精子症的女性中，有一半的人阴道加德纳菌占优势，但在伴侣没有白细胞精子症的17名女性中，只有一人是阴道加德纳菌。性交后皱纹乳杆菌的相对丰度显著下降，精液与阴道标本的一致性较高。我们的数据支持精液和阴道微生物群相关的假说，因为阴道毛滴虫在女性伴侣中的优势与男性生殖道的炎症显著相关。(C)2015年巴斯德研究所。爱思唯尔·马森公司出版。版权所有。
The genital tract microbiome is tightly associated with reproductive health. Although many research studies have been performed on the vaginal microbiome, current knowledge of the male microbiome is scarce, and parallel studies examining couples are extremely rare. In this work, we aimed to compare seminal and vaginal microbiomes in couples and to assess the influence of sexual intercourse on vaginal microbiome.The study included 23 couples. Microbiomes of semen and vaginal fluid (pre- and post-intercourse) were profiled using Illumina HiSeq2000 sequencing of the V6 region of 16S rRNA gene.Seminal communities were significantly more diverse, but with lower total bacterial concentrations than those of the vagina. Gardnerella vaginalis was predominant in half of the women whose partners had significant leukocytospermia, but only in one of 17 women who had a partner without leukocytospermia. There was significant decrease in the relative abundance of Lactobacillus crispatus after intercourse, and high concordance between semen and vaginal samples. Our data support the hypothesis that semen and vaginal microbiomes are in association, inasmuch as the predominance of G. vaginalis in female partners was significantly related to inflammation in male genital tracts. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.