Molecular Dissection of α‐ and Dense‐Core Granule Secretion of Platelets
Molecular Dissection of α‐ and Dense‐Core Granule Secretion of Platelets
复制标题
血小板α-和致密核心颗粒分泌的分子解剖
DOI：
10.1111/j.1749-6632.2001.tb03973.x
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发表时间：
2001
影响因子：
5.2
通讯作者：
T. Kita
中科院分区：
文献类型：
--
作者：
A. Yoshioka;H. Horiuchi;R. Shirakawa;H. Nishioka;A. Tabuchi;T. Higashi;A. Yamamoto;T. Kita
摘要：血小板被激活后，会释放许多储存在α颗粒和致密核颗粒中的活性物质。然而，调控胞吐作用的分子机制尚不完全清楚。我们建立了一种利用链溶酶-O通透性的血小板来分析钙离子诱导的α颗粒中储存的von Willebrand因子和致密核颗粒中的[~3H]5-羟色胺(5-HT)的分泌的检测系统。利用该方法，我们发现小分子GTP酶Rab4能调节血小板内α-，但不能调节致密核颗粒的分泌。此外，我们还纯化了一个胞质必需蛋白，目前正在对其功能进行分析。
Abstract: Upon activation, platelets release many active substances stored in α‐ and dense‐core granules. However, the molecular mechanisms governing the regulated exocytosis are not yet fully understood. We have established an assay system using streptolysin‐O‐permeabilized platelets to analyze the Ca2+‐induced secretions of von Willebrand factor stored in α‐granules and [3H]5‐hydroxytryptamine (5‐HT) in dense‐core granules. Using the assay, we found that small GTPase Rab4 regulates α‐, but not dense‐core, granule secretion in platelets. Furthermore, we purified a cytosolic essential protein and currently are analyzing its function.