Action spectrum of retinal light-damage in albino rats.
Action spectrum of retinal light-damage in albino rats.
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DOI：
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发表时间：
1983-03
影响因子：
4.4
通讯作者：
T. P. Williams;W. L. Howell
中科院分区：
文献类型：
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作者：
T. P. Williams;W. L. Howell
将麻醉的10周龄白化病大鼠的右眼暴露于6种波长的恒定光子通量6小时。在暴露期间，将每只动物的左眼贴上眼罩，并用作对照。视网膜切片的组织学检查揭示了上级视网膜的一个区域比其他区域受损更严重。试图通过测量在这个“敏感”区域丢失的ONL来确定作用谱失败了。然而，当ONL厚度在整个视网膜切片上积分时，显示出视紫红质作用谱。得出的结论是：（1）在这些条件下，白化病大鼠的视网膜光损伤是视紫红质介导的，（2）损伤程度的正确评估只能通过某种方法，整合整个视网膜切片。
The right eyes of anesthetized, 10-week-old albino rats were exposed to constant photon fluxes at 6 wavelengths for 6 hrs. The left eye of each animal was patched during the exposure and was used as control. Histologic examination of retinal sections disclosed a region in the superior retina that was more damaged than were other areas. Attempting to ascertain an action spectrum by measuring ONL lost in this "sensitive" region failed. However, it was shown that, when ONL thickness was integrated over the entire retinal sections, a rhodopsin action-spectrum emerged. It was concluded that (1) retinal light damage in the albino rat under these conditions was rhodopsin mediated, and (2) proper assessment of the extent of damage could only be made by some method that integrates over the entire retinal section.