Morphologic integration of hilar ectopic granule cells into dentate gyrus circuitry in the pilocarpine model of temporal lobe epilepsy.
Morphologic integration of hilar ectopic granule cells into dentate gyrus circuitry in the pilocarpine model of temporal lobe epilepsy.
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DOI：
10.1002/cne.22623
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发表时间：
2011-08-01
影响因子：
2.5
通讯作者：
Nadler, J. Victor
中科院分区：
文献类型：
--
作者：
Cameron, Michael C.;Zhan, Ren-Zhi;Nadler, J. Victor
在匹罗卡品诱导的癫痫持续状态后，许多颗粒细胞在癫痫发作后的环境中异常迁移到齿状门。肺门异位颗粒细胞（HEGCs）是超兴奋的，因此可能会增加电路的兴奋性。本研究确定了它们的轴突和树突的分布。在匹罗卡品处理的大鼠海马脑片的全细胞膜片钳记录过程中，HEGCs和正常运动颗粒细胞被生物胞素填充。86%的生物胞素标记的HEGCs的顶端树突延伸到齿状分子层的外缘。HEGC顶树突的总长度和分支穿透分子层显着减少相比，顶树突的正常定位颗粒细胞。HEGCs比正常定位颗粒细胞更可能具有门基底树突。它们与正常定位颗粒细胞一样可能投射到切片内的CA3锥体细胞，但更可能将至少一个复发苔藓纤维送入分子层。具有爆发能力的HEGCs具有较少分支的顶端树突，比nonbursting HEGCs，其树突更可能局限于门部，并且一些表现出与未成熟颗粒细胞相似的树突特征。因此，HEGC有许多沿着的路径，从正常运动颗粒细胞接收同步活动，并将其自身的过度活动传递给正常运动颗粒细胞和CA 3锥体细胞。因此，它们可能有助于高度互连的颗粒细胞枢纽，这些枢纽被认为对过度兴奋、潜在的易兴奋回路的发展至关重要。
After pilocarpine-induced status epilepticus, many granule cells born into the postseizure environment migrate aberrantly into the dentate hilus. Hilar ectopic granule cells (HEGCs) are hyperexcitable and may therefore increase circuit excitability. This study determined the distribution of their axons and dendrites. HEGCs and normotopic granule cells were filled with biocytin during whole-cell patch clamp recording in hippocampal slices from pilocarpine-treated rats. The apical dendrite of 86% of the biocytin-labeled HEGCs extended to the outer edge of the dentate molecular layer. The total length and branching of HEGC apical dendrites that penetrated the molecular layer were significantly reduced compared with apical dendrites of normotopic granule cells. HEGCs were much more likely to have a hilar basal dendrite than normotopic granule cells. They were about as likely as normotopic granule cells to project to CA3 pyramidal cells within the slice, but were much more likely to send at least one recurrent mossy fiber into the molecular layer. HEGCs with burst capability had less well-branched apical dendrites than nonbursting HEGCs, their dendrites were more likely to be confined to the hilus, and some exhibited dendritic features similar to those of immature granule cells. HEGCs thus have many paths along which to receive synchronized activity from normotopic granule cells and to transmit their own hyperactivity to both normotopic granule cells and CA3 pyramidal cells. They may therefore contribute to the highly interconnected granule cell hubs that have been proposed as crucial to development of a hyperexcitable, potentially seizure-prone circuit.
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影响因子：
2.5
作者：
Liu, YB;Lio, PA;Trommer, BL
通讯作者：
Trommer, BL
影响因子：
2.5
作者：
Buckmaster, PS;Dudek, FE
通讯作者：
Dudek, FE
影响因子：
6.1
作者：
Jung, Keun-Hwa;Chu, Kon;Roh, Jae-Kyu
通讯作者：
Roh, Jae-Kyu
影响因子：
5.3
作者：
Kron, Michelle M.;Zhang, Helen;Parent, Jack M.
通讯作者：
Parent, Jack M.
影响因子：
5.6
作者：
Engel, J
通讯作者：
Engel, J