Inhibitory effects of sword bean extract on alveolar bone resorption induced in rats by Porphyromonas gingivalis infection.
Inhibitory effects of sword bean extract on alveolar bone resorption induced in rats by Porphyromonas gingivalis infection.
复制标题
剑豆提取物对牙龈卟啉单胞菌感染引起的大鼠牙槽骨吸收的抑制作用。
DOI：
10.1111/jre.12166
复制
发表时间：
2014
影响因子：
3.5
通讯作者：
Furuichi Y.
中科院分区：
文献类型：
--
作者：
Nakatsuka Y;Nagasawa T;Yumoto Y;Nakazawa F;Furuichi Y.
驯化的豆科植物——剑豆（Canavalia gladiata）（通常被称为剑豆）含有大麻碱。这种水果在中国和日本的草药中用于治疗脓液的排出，但其药理机制尚不清楚。目的研究剑豆提取物（SBE）对口腔细菌和人口腔上皮细胞的体外影响，以及实验性牙龈卟啉单胞菌诱导的大鼠牙槽骨吸收的开始和进展。材料与方法采用高效液相色谱/紫外分光光度法定量测定SBE中大麻碱的含量。通过对口腔细菌生长情况的评估，估计出SBE、菜豆氨酸、葡萄糖酸氯己定（CHX）溶液的最小抑菌浓度和最小杀菌浓度。采用台盼蓝法测定SBE、菜豆碱、CHX、胰肽和胱抑素对KB细胞的细胞毒性。采用合成底物，采用比色法测定SBE、菜豆氨酸、胰肽和胱抑素对Arg‐gingipain （Rgp）和Lys‐gingipain （Kgp）的影响。检验其对p的影响。与牙龈相关的牙周组织破裂，SBE口服toP。gingivalis所致感染的老鼠。结果剑豆提取物中含有6.4%的大麻碱。SBE和大麻碱抑制了p的生长。gingivalisandFusobacterium nucleatum。SBE、canavanine和cystatin对KB细胞的细胞毒性显著低于CHX。SBE对Rgp的抑制作用与已知Rgp抑制剂lepeptin相当，且在500 μg/mL浓度下，SBE对Kgp的抑制作用显著高于lepeptin （p<0.05）。给药SBE显著抑制牙龈诱导的牙槽骨吸收，其骨水平与未感染动物相当（p<0.05）。结论SBE对stp有一定的治疗作用。牙龈相关的牙槽骨吸收。
BackgroundThe domesticated legume,Canavalia gladiata(commonly called the sword bean), is known to contain canavanine. The fruit is used in Chinese and Japanese herbal medicine for treating the discharge of pus, but its pharmacological mechanisms are still unclear.ObjectivesThis study examined the effect of sword bean extract (SBE) on (i) oral bacteria and human oral epithelial cellsin vitro, and (ii) the initiation and progression of experimentalPorphyromonas gingivalis‐induced alveolar bone resorption in rats.Material and MethodsA high‐performance liquid chromatography/ultraviolet method was applied to quantitate canavanine in SBE. By assessing oral bacterial growth, we estimated the minimum inhibitory concentration and minimum bactericidal concentration of SBE, canavanine, chlorhexidine gluconate (CHX) solution. The cytotoxicity of SBE, canavanine, CHX, leupeptin and cystatin for KB cells was determined using a trypan blue assay. The effects of SBE, canavanine, leupeptin and cystatin on Arg‐gingipain (Rgp) and Lys‐gingipain (Kgp) were evaluated by colorimetric assay using synthetic substrates. To examine its effects onP. gingivalis‐associated periodontal tissue breakdown, SBE was orally administered toP. gingivalis‐infected rats.ResultSword bean extract contained 6.4% canavanine. SBE and canavanine inhibited the growth ofP. gingivalisandFusobacterium nucleatum. The cytotoxicity of SBE, canavanine and cystatin on KB cells was significantly lower than that of CHX. Inhibition of Rgp with SBE was comparable to that with leupeptin, a known Rgp inhibitor, and inhibition of Kgp with SBE was significantly higher than that with leupeptin at 500 μg/mL (p<0.05).P. gingivalis‐induced alveolar bone resorption was significantly suppressed by administration of SBE, with bone levels remaining comparable to non‐infected animals (p<0.05).ConclusionThe present study suggests that SBE might be effective againstP. gingivalis‐associated alveolar bone resorption.