A cluster of 11 CBF transcription factors is located at the frost tolerance locus Fr-Am2 in Triticum monococcum
A cluster of 11 CBF transcription factors is located at the frost tolerance locus Fr-Am2 in Triticum monococcum
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DOI：
10.1007/s00438-005-0076-6
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发表时间：
2006-02-01
影响因子：
3.1
通讯作者：
Dubcovsky, J
中科院分区：
文献类型：
--
作者：
Miller, AK;Galiba, G;Dubcovsky, J
由于低温对冬小麦的不利影响，抗冻性是严冬地区育种计划的重要性状。Fr-A（m）2是近年来在二倍体小麦中发现的耐霜冻基因座。该基因座作为QTL定位在染色体5A（m）上，与冷调节基因COR 14 b在15 ℃下的转录水平的QTL位于同一区域。CBF转录因子定位在这两个重叠QTL的中心。然而，由于小麦中的CBF基因家族具有众多成员，因此可能在Fr-A（m）2处存在多个CBF基因。为了研究这种可能性，我们对T.单球菌属在这里，我们报告了13个TmCBF基因的分子特征。其中9个根据最接近的大麦HvCBF基因进行编号，另外4个没有明确的大麦直向同源物的编号为TmCBF 15至TmCBF 18。TmCBF 5和TmCBF 18在T.单球菌染色体7A（m）和6A（m），因此不是Fr-A（m）2基因的候选者。其余11个TmCBF基因在5个不同的细菌人工染色体（BAC）克隆中聚集在Fr-A（m）2位点。使用高密度图谱对这些BAC进行了定位，并且在大多数BAC之间发现了重组事件。携带这些重组事件的株系将有助于鉴定哪一个CBF基因负责T.在Fr-A（m）2基因座处，
Due to the adverse effects of cold temperatures on winter wheat, frost tolerance is an important trait for breeding programs in regions with severe winters. Frost tolerance locus Fr-A(m)2 was recently discovered in diploid wheat (Triticum monococcum L.). This locus was mapped as a QTL on chromosome 5A(m) in the same region as a QTL for the level of transcription of the cold-regulated gene COR14b at 15 degrees C. A CBF transcription factor was mapped in the center of these two overlapping QTLs. However, since the CBF gene family in wheat has numerous members, it was possible that multiple CBF genes were present at Fr-A(m)2. To investigate this possibility we initiated a systematic characterization of the CBF family in T. monococcum. Here we report the molecular characterization of thirteen TmCBF genes. Nine of them were numbered according to the closest barley HvCBF gene, and the other four that have no clear barley orthologues were assigned numbers TmCBF15 to TmCBF18. TmCBF5 and TmCBF18 were mapped on T. monococcum chromosomes 7A(m) and 6A(m), respectively, and are thus not candidates for the Fr-A(m)2 gene. The remaining eleven TmCBF genes are clustered at the Fr-A(m)2 locus within five different Bacterial Artificial Chromosome (BAC) clones. These BACs were mapped using a high-density map and recombination events were found between most BACs. Lines carrying these recombination events will be useful to identify which of the CBF genes is responsible for the differences in frost tolerance between the T. monococcum parental lines at the Fr-A(m)2 locus.