MODULATION OF HERPES-SIMPLEX VIRUS TYPE-1 REPLICATION BY HUMAN SALIVARY SECRETIONS
MODULATION OF HERPES-SIMPLEX VIRUS TYPE-1 REPLICATION BY HUMAN SALIVARY SECRETIONS
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DOI：
10.1111/j.1399-302x.1993.tb00551.x
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发表时间：
1993-04-01
影响因子：
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通讯作者：
LEVINE, MJ
中科院分区：
文献类型：
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作者：
BERGEY, EJ;GU, M;LEVINE, MJ
唾液通过调节微生物在口腔表面定殖的能力或限制其生长和/或活力来保护口腔免受病原体入侵。尽管唾液分泌物在调节口腔细菌菌群中的作用受到了相当多的关注，但对其在病毒发病机制中的作用却知之甚少。因此，本研究的目的是评估唾液分泌物对 1 型单纯疱疹病毒 (HSV-1) 复制的影响。最初，进行 HSV-1 噬菌斑和滴度降低测定，以确定人颌下/舌下 (HSMSL) 和腮腺 (HPS) 唾液抑制 HSV-1 感染早期阶段（吸附和渗透）的能力。我们的结果表明HSMSL和HPS都具有细胞保护和病毒中和活性，其中HSMSL比HPS更活跃。进行了额外的实验以确定唾液对病毒后代产量的影响。同样，HSMSL 比 HPS 更能减少 HSV-1 复制。使用牛痘无法获得类似的效果，这表明人类唾液的这种抑制活性是选择性的。总的来说，这些结果表明人类唾液分泌物可以在体外调节 HSV-1 的复制。
Saliva functions to protect the oral cavity from pathogenic invasion by modulating the ability of microbes to colonize the oral surfaces or limiting their growth and/or viability. Although the role of salivary secretions in the modulation of the oral bacteria flora has received considerable attention, little is known concerning its role in viral pathogenesis. Accordingly, the purpose of this study was to assess the effect of salivary secretions on herpes simplex virus type 1 (HSV-1) replication. Initially, HSV-1 plaque and titer reduction assays were performed to determine the ability of human submandibular/sublingual (HSMSL) and parotid (HPS) salivas to inhibit the early stages of HSV-1 infection (adsorption and penetration). Our results suggested that both HSMSL and HPS possess cell-protective and virus neutralization activities, with HSMSL being more active than HPS. Additional experiments were performed to determine the effect of saliva on the yield of virus progeny. Again, HSMSL caused a greater reduction of HSV-1 replication than did HPS. A similar effect could not be obtained using vaccinia, suggesting that this inhibitory activity of human saliva is selective. Collectively, these results suggest that human salivary secretions can modulate the replication of HSV-1 in vitro.