Developments in Mass Spectrometry for Glycosaminoglycan Analysis: A Review.
Developments in Mass Spectrometry for Glycosaminoglycan Analysis: A Review.
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DOI：
10.1074/mcp.r120.002267
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Amster IJ
中科院分区：
文献类型：
--
作者：
Pepi LE;Sanderson P;Stickney M;Amster IJ
本文综述了近年来糖胺多聚糖(GAG)的质谱学分析研究进展。GAG参与多种生物学功能，包括细胞通讯、伤口愈合和抗凝，是结构表征的重要靶点。由于可能发生的各种O-和N-硫化修饰以及糖醛酸C-5异构化，GAG显示出不同的结构特征，这使得它们的分析成为一个具有挑战性的目标。GAG结构指认的质谱学方法已经得到了广泛的研究，新的方法仍然是开发的主题。样品制备、串联MS技术(MS/MS)、在线分离和自动分析软件的进步推动了GAG分析领域的发展。这些最新的发展导致了GAG结构表征的精度和时间效率的显着改进。串联质谱仪在线分离可对糖胺多聚糖进行测序。电子激发增加了碰撞激活时的GAG序列覆盖率。这种方法对于生物样品和药物的分析是有用的。糖胺多聚糖(GAG)参与多种生物学功能，具有多种药用价值。由于其非模板驱动的生物合成，GAG以不均匀的复杂混合物的形式产生。质谱学结合在线分离技术已被用来确定这些复杂混合物的组成。串联质谱学的进步也使得确定序列信息，如硫化位置和C-5差向异构化成为可能。本文综述了近年来GAG的质谱学分析研究进展。
This review covers recent developments in glycosaminoglycan (GAG) analysis via mass spectrometry (MS). GAGs participate in a variety of biological functions, including cellular communication, wound healing, and anticoagulation, and are important targets for structural characterization. GAGs exhibit a diverse range of structural features due to the variety of O- and N-sulfation modifications and uronic acid C-5 epimerization that can occur, making their analysis a challenging target. Mass spectrometry approaches to the structure assignment of GAGs have been widely investigated, and new methodologies remain the subject of development. Advances in sample preparation, tandem MS techniques (MS/MS), online separations, and automated analysis software have advanced the field of GAG analysis. These recent developments have led to remarkable improvements in the precision and time efficiency for the structural characterization of GAGs. Online separation with tandem mass spectrometry can sequence glycosaminoglycans. Electronic excitation increases GAG sequence coverage over collisional activation. This approach is useful for analysis of biological samples and pharmaceuticals. Glycosaminoglycans (GAGs) participate in a variety of biological functions and have a multitude of medicinal properties. Due to their non template driven biosynthesis, GAGs are produced as nonuniform complex mixtures. Mass spectrometry paired with on-line separation techniques has been utilized to determine the composition of these complex mixtures. Advances in tandem mass spectrometry have also made determining sequence information such as sulfation location and C-5 epimerization possible. This review covers recent developments in the analysis of GAGs using mass spectrometry.
蓝鲨 (Prionace glauca) 软骨中高度硫酸化的角质素硫酸盐的结构表征及其与 RCA(120) 的相互作用
影响因子：
5.4
作者：
Li Q;Li G;Zhao X;Shan X;Cai C;Zhao J;Zhang F;Linhardt RJ;Yu G
通讯作者：
Yu G