Increased apoptosis and cysteinyl aspartate specific protease-3 gene expression in human intracranial aneurysm
Increased apoptosis and cysteinyl aspartate specific protease-3 gene expression in human intracranial aneurysm
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DOI：
10.1016/j.jocn.2005.11.018
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发表时间：
2007-06-01
影响因子：
2
通讯作者：
You, Chao
中科院分区：
文献类型：
--
作者：
Guo, Fuyou;Li, Zhihua;You, Chao
目的：目的：探讨破裂颅内动脉瘤血管平滑肌细胞（VSMCs）凋亡及caspase-3表达的意义。方法：对15例破裂颅内动脉瘤、6例腹主动脉瘤（AAA）及6例正常血管进行检测。透射电镜观察细胞凋亡情况。采用α-SMC肌动蛋白的免疫组织化学和直接细胞计数（每高倍视野（HPF）的中膜VSMC）来确定中膜VSMC密度。此外，caspase-3的基因表达测定使用real-time RT-PCR.Results：我们证明中膜VSMCs表现出形态学凋亡的变化，在脑动脉瘤和AAA。与正常动脉（222.8 +/- 12.1 SMC/HPF-，p < 0.01）相比，颅内动脉瘤（43.9 +/- 4.3 SMC/HPF）和AAA（53.2 +/- 9.4 SMC/HPF）的中膜VSMC密度显著降低。Caspase-3 mRNA在颅内动脉瘤和AAA中的表达分别是正常血管的8.94倍和6.73倍。结论：真实的时间RT-PCR为检测小样本中基因表达提供了一种有用的工具，有助于更好地了解凋亡在颅内动脉瘤破裂中的作用。(c)2005爱思唯尔有限公司保留所有权利。
Objective: To investigate apoptosis in vascular smooth muscle cells (VSMCs) and caspase-3 expression in ruptured intracranial aneurysm.Methods: Tissue samples of 15 ruptured intracranial aneurysms, 6 abdominal aortic aneurysms (AAA) and 6 normal vessels were evaluated. Apoptosis in VSMCs was determined on transmission electron microscopy. Immunohistochemistry for alpha-SMC actin and direct cell counts (medial VSMCs per high-power field (HPF)) were employed to determine medial VSMC density. Additionally, gene expression of caspase-3 was determined using real-time RT-PCR.Results: We demonstrated medial VSMCs exhibiting morphological apoptotic changes in cerebral aneurysm and AAA. Medial VSMC density was significantly decreased in intracranial aneurysm (43.9 +/- 4.3 SMCs/HPF) and AAA (53.2 +/- 9.4 SMCs/HPF) compared with the normal arteries (222.8 +/- 12.1 SMCs/HPF-, p < 0.01). An 8.94-fold and 6.73-fold increase in expression of caspase-3mRNA in intracranial aneurysm and AAA, respectively, were obtained relative to the normal vessels.Conclusions: These results suggest that real time RT-PCR provides a useful tool to test gene expression in small samples, and may contribute to a better understanding of the role of apoptosis in ruptured intracranial aneurysm. (c) 2005 Elsevier Ltd. All rights reserved.