The promoter of the Arabidopsis thaliana BAN gene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coat
The promoter of the Arabidopsis thaliana BAN gene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coat
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DOI：
10.1007/s00299-008-0667-x
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发表时间：
2009-04-01
影响因子：
6.2
通讯作者：
Renard, Michel
中科院分区：
文献类型：
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作者：
Nesi, Nathalie;Lucas, Marie-Odile;Renard, Michel
作为一个正在进行的研究计划，致力于了解油菜种皮中的原花青素（PA）的积累，转基因油菜植物携带的2.3 kb片段的拟南芥BAN启动子（ProAtBAN）融合的uidA报告基因（GUS）的产生。对这些植物的分析表明，ProAtBAN在B中被激活。油菜种皮中的PA沉积模式，这是非常相似的油菜籽中的PA沉积曲线，也是一个先前描述的拟南芥的时空模式。ProAtBAN活性发生在胚胎发生的早期阶段，并仅限于PA积累的细胞。因此，拟南芥BAN启动子可用于触发B中的基因表达。油菜种皮用于基因工程和候选基因的功能验证。此外，这些数据有力地表明，BAN基因的转录调控网络是保守的拟南芥和油菜。这与公共油菜籽序列数据库的相似性搜索允许回收先前在拟南芥中描述的几种BAN调节剂（即TT1、TT2、TT8、TT16和TTG1）的油菜籽同源物的事实一致。
As part of an ongoing research program dedicated to the understanding of proanthocyanidin (PA) accumulation in Brassica napus seed coat, transgenic rapeseed plants carrying a 2.3-kb fragment of the Arabidopsis thaliana BAN promoter (ProAtBAN) fused to the uidA reporter gene (GUS) were generated. Analysis of these plants revealed that ProAtBAN was activated in B. napus seed coat, following a spatio-temporal pattern that was very similar to the PA deposition profile in rapeseed and also to the one previously described in Arabidopsis. ProAtBAN activity occurred as soon as the early stages of embryogenesis and was restricted to the cells where PAs were shown to accumulate. Therefore, the Arabidopsis BAN promoter can be used to trigger gene expression in B. napus seed coat for both genetic engineering and functional validation of candidate genes. In addition, these data strongly suggest that the transcriptional regulatory network of the BAN gene is conserved between Arabidopsis and rapeseed. This is consistent with the fact that similarity searches of the public rapeseed sequence databases allowed recovering the rapeseed homologs for several BAN regulators, namely TT1, TT2, TT8, TT16 and TTG1, which have been previously described in Arabidopsis.