Transcription Factors Expressed in Mouse Cochlear Inner and Outer Hair Cells.
Transcription Factors Expressed in Mouse Cochlear Inner and Outer Hair Cells.
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DOI：
10.1371/journal.pone.0151291
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
He DZ
中科院分区：
文献类型：
--
作者：
Li Y;Liu H;Barta CL;Judge PD;Zhao L;Zhang WJ;Gong S;Beisel KW;He DZ
基因表达的调节对于确定不同细胞之间的功能复杂性和形态多样性至关重要。转录调控是基因表达调控的关键步骤，因为遗传信息是由序列特异性转录因子（TF）直接从DNA中读取的。虽然几个小鼠TF数据库创建的基因组序列和转录组是可用的，细胞类型特异性TF数据库从任何正常细胞群体仍然缺乏。我们使用成年小鼠的毛细胞特异性转录组鉴定了耳蜗内毛细胞（IHCs）和外毛细胞（OHCs）中表达的细胞类型特异性TF基因。内毛细胞和外毛细胞是哺乳动物耳蜗中的两种感觉感受细胞。我们发现，在2,230个推定的小鼠TF基因中，分别有1,563个和1,616个TF基因在IHC和OHC中表达。虽然1，536个基因在两个人群中普遍表达，但73个基因在IHC中差异表达（至少有两倍差异），13个基因在OHC中差异表达。我们的数据集代表了两个感觉受体细胞群体的第一个细胞类型特异性TF数据库，并且是理解这些细胞的生物学特性和表型差异的分子机制的关键信息资源。
Regulation of gene expression is essential to determining the functional complexity and morphological diversity seen among different cells. Transcriptional regulation is a crucial step in gene expression regulation because the genetic information is directly read from DNA by sequence-specific transcription factors (TFs). Although several mouse TF databases created from genome sequences and transcriptomes are available, a cell type-specific TF database from any normal cell populations is still lacking. We identify cell type-specific TF genes expressed in cochlear inner hair cells (IHCs) and outer hair cells (OHCs) using hair cell-specific transcriptomes from adult mice. IHCs and OHCs are the two types of sensory receptor cells in the mammalian cochlea. We show that 1,563 and 1,616 TF genes are respectively expressed in IHCs and OHCs among 2,230 putative mouse TF genes. While 1,536 are commonly expressed in both populations, 73 genes are differentially expressed (with at least a twofold difference) in IHCs and 13 are differentially expressed in OHCs. Our datasets represent the first cell type-specific TF databases for two populations of sensory receptor cells and are key informational resources for understanding the molecular mechanism underlying the biological properties and phenotypical differences of these cells.