Electrochemical sensors-based phosphorus-doped carbon for determination of adenine DNA-nucleobases in living cells
Electrochemical sensors-based phosphorus-doped carbon for determination of adenine DNA-nucleobases in living cells
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DOI：
10.1016/j.carbon.2020.10.041
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发表时间：
2021-01-04
期刊：
影响因子：
10.9
通讯作者：
Shenashen, Mohamed A.
中科院分区：
文献类型：
--
作者：
Emran, Mohammed Y.;El-Safty, Sherif A.;Shenashen, Mohamed A.
监测活细胞中的DNA-核碱基及其氧化形式被认为是DNA损伤的生物标志物。设计了一种基于磷掺杂碳球眼结构（P-CES）的腺嘌呤电化学传感器。球形电化学传感器具有丰富的多个定向门，如微孔、中孔和开放的洞穴样眼，可以设计具有快速响应、低表面电荷电阻、高电子扩散和功能化结合活性中心以容纳Ad分子的潜在传感器。P-CES分级有序的球形电极具有密集和反应性暴露部位，用于在Ad检测期间进行各种额外的相互作用和灵活的移动表面。具有粗糙表面、多孔结构和开放凹槽的圆形形状的关键作用是产生与目标分子强结合的活性催化剂，并促进电极表面内/外的分子扩散。此外，P原子掺杂到分级碳链中导致sp（2）-石墨结构的畸变，增加了平面边缘缺陷的数量，并诱导了丰富活性中心的功能化。因此，P-CES提供了用于检测其资源（活细胞中的氧化DNA）中的Ad的活性转导元件，其具有高灵敏度、良好的选择性、优异的稳定性和容易的再现性。所制备的P-CES显示出超灵敏的Ad传感器，其检测限低至0.002 μ molL（-1），线性范围为0.01-0.8 mmolL（-1）。从活细胞中的氧化-DNA释放的Ad可以以高亲和力和灵敏度检测。Ad传感器检测可用于DNA损伤的研究，并为细胞突变和免疫系统缺陷引起的疾病的诊断提供信息。(C)2020爱思唯尔有限公司保留所有权利。
Monitoring DNA-nucleobases and their oxidized forms in living cells is regarded as a biomarker for DNA damage. Adenine (Ad) electrochemical sensor was designed based on phosphorus-doped carbon spherical eye-like structure (P-CES). Spherical electrochemical sensors oriented with abundant multiple directional gates, such as micro-, meso-pores, and open cave-like eyes may lead to design a potential sensor with fast response, low surface charge resistance, high electron diffusion, and functionalized binding active centers to accommodate Ad molecules. The P-CES hierarchically ordered sphere electrode features dense and reactive exposure sites for a variety of additional interactions and flexible mobility surfaces during the detection of Ad. The key role of the circular shape with a rugged surface, multi-hole construction, and open grooves creates an active catalyst of strong binding to the target molecules and facilitate the molecular diffusion inner/outer the electrode surface. Moreover, the doping of P atoms to the hierarchical carbon chain leads to the distortion of sp(2)-graphitic construction, increases the number of plane edge defects, and induces the functionalization of the abundant active sites. Thus, P-CES provides active transducing element for detection of Ad in its resources (oxidative DNA in living cells) with high sensitivity, good selectivity, excellent stability, and facile reproducibility. The fabricated P-CES shows an ultrasensitive Ad sensor with a limit of detection as low as 0.002 mu molL(-1), with a wide linear range of 0.01-0.8 mmolL(-1). The Ad liberated from the oxidative-DNA in living cells can be detected with high affinity and sensitivity. The Ad sensor assay can be employed for investigation of DNA damage and deliver information for diagnosis of diseases caused by cell mutation and immune system deficiency. (C) 2020 Elsevier Ltd. All rights reserved.