Confocal fluorescence spectroscopy and anisotropy imaging system
Confocal fluorescence spectroscopy and anisotropy imaging system
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DOI：
10.1364/ol.28.000695
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发表时间：
2003-05-01
期刊：
影响因子：
3.6
通讯作者：
Foster, TH
中科院分区：
文献类型：
--
作者：
Bigelow, CE;Conover, DL;Foster, TH
我们报告的设计和实施的激光扫描共聚焦荧光系统的光谱和各向异性成像能力。共焦光谱是通过光纤针孔实现的，该光纤针孔根据需要插入检测路径中或从检测路径中移除。荧光各向异性成像是用放置在常规针孔之后的偏振分束器来完成的。用平衡光电倍增管同时检测两个正交偏振。在共聚焦荧光光谱模式中实现的轴向切片的质量通过实验证明，并且在肿瘤细胞单层中证明了偏振敏感荧光成像的示例。(C)2003年美国光学学会。
We report the design and implementation of a laser scanning confocal fluorescence system with spectroscopy and anisotropy imaging capabilities. Confocal spectroscopy is achieved with a fiber pinhole that is inserted into and removed from the detection path as needed. Fluorescence anisotropy imaging is accomplished with a polarizing beam splitter placed after the conventional pinhole. Two orthogonal polarizations are detected simultaneously with balanced photomultiplier tubes. The quality of the axial sectioning that is achieved in the confocal fluorescence spectroscopy mode is demonstrated experimentally, and examples of polarization-sensitive fluorescence imaging are demonstrated in tumor cell monolayers. (C) 2003 Optical Society of America.