Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body
Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body
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DOI：
10.1016/s1534-5807(02)00219-8
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发表时间：
2002-08-01
影响因子：
11.8
通讯作者：
Emr, SD
中科院分区：
文献类型：
--
作者：
Babst, M;Katzmann, DJ;Emr, SD
将泛素化的内体膜蛋白分选到MVB途径中由E类Vps蛋白复合物ESCRT-I、-II和-III以及AAA型ATP酶Vps 4执行。本研究表征了ESCRT-II，一种由E类Vps蛋白Vps 22、Vps 25和Vps 36形成的可溶性类似于155 kDa蛋白复合物。该蛋白质复合物与内体膜瞬时结合，从而启动ESCRT-III的形成，ESCRT-III是一种膜结合蛋白质复合物，在MVB货物分选期间在ESCRT-II的下游立即起作用。ESCRT-II反过来在ESCRT-I的下游起作用，ESCRT-I是一种结合泛素化内体货物的蛋白质复合物。我们建议，ESCRT复合物执行协调级联的事件，以选择和排序MVB货物交付到腔的空泡/溶酶体。
Sorting of ubiquitinated endosomal membrane proteins into the MVB pathway is executed by the class E Vps protein complexes ESCRT-I, -II, and -III, and the AAA-type ATPase Vps4. This study characterizes ESCRT-II, a soluble similar to155 kDa protein complex formed by the class E Vps proteins Vps22, Vps25, and Vps36. This protein complex transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo. We propose that the ESCRT complexes perform a coordinated cascade of events to select and sort MVB cargoes for delivery to the lumen of the vacuole/lysosome.