Kinesin heads fused to hinge-free myosin tails drive efficient motility
Kinesin heads fused to hinge-free myosin tails drive efficient motility
复制标题
DOI：
10.1016/j.febslet.2004.05.050
复制
发表时间：
2004-07-02
期刊：
影响因子：
3.5
通讯作者：
Cross, R
中科院分区：
文献类型：
--
作者：
Eickel, V;Drummond, D;Cross, R
大鼠驱动蛋白运动域分别在残基433、411、376或367处融合至刷状缘肌球蛋白尾的C-末端1185、1187、1197或1185残基。在运动性实验中，K433 myt和K411 myt保留了头端驱动蛋白铰链，而K367 myt将其删除，它们驱动微管快速滑动（近似于0.6 mums（-1）），当头端对通过添加1：1无头肌球蛋白尾部而间隔开时，这种滑动是最佳的。部分删除头部近端铰链的K376 myt在滑动试验中表现出较差的运动性，但在单分子光学捕获中表现出野生型的持续合成能力、速度和失速力。因此，头近端驱动蛋白铰链在功能上是不稳定的。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The rat kinesin motor domain was fused at residues 433, 411, 376 or 367, respectively, to the C-terminal 1185, 1187, 1197 or 1185 residues of the brush border myosin tail. In motility assays, K433myt and K411myt, which preserve the head-proximal kinesin hinge, and K367myt, which deletes it, drove rapid microtubule sliding (similar to0.6 mums(-1)) that was optimal when the head-pairs were spaced apart by adding 1:1 headless myosin tails. K376myt, which partially deletes the head-proximal hinge, showed poor motility in sliding assays but wild type processivity, velocity and stall force in single molecule optical trapping. Accordingly, the head-proximal kinesin hinge is functionally dispensable. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.