HLA antigens in colorectal tumours--low expression of HLA class I antigens in mucinous colorectal carcinomas.
HLA antigens in colorectal tumours--low expression of HLA class I antigens in mucinous colorectal carcinomas.
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结直肠肿瘤中的 HLA 抗原——粘液性结直肠癌中 HLA I 类抗原的低表达。
DOI：
10.1038/bjc.1987.26
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发表时间：
1987
影响因子：
8.8
通讯作者：
Ferrone,S
中科院分区：
文献类型：
--
作者：
vandenIngh,HF;Ruiter,DJ;Griffioen,G;vanMuijen,GN;Ferrone,S
用单抗对9例粘液性和10例非粘液性结直肠癌、1例泄殖性结直肠癌、12例结直肠腺瘤和4例正常大肠黏膜冰冻切片进行了人类白细胞抗原和β2-微球蛋白的免疫组织化学染色。染色结果与组织病理学特征相关。在形态正常的大肠上皮、所有的腺瘤和所有的非粘液性癌中，HL A-I类抗原都有较强的表达。相反，在9例粘液性癌中只有2例肿瘤细胞表达HLAI类抗原，而这些粘液性癌中有2例完全阴性。在粘液性癌中，单核细胞炎性浸润物明显稀少，尤其是粘液堆积周围。12例腺瘤中仅1例局灶性表达HL A-II类抗原，正常上皮未见表达。在所检测的20例肿瘤中，有6例肿瘤细胞呈抗HLAII类抗原的单抗染色，阳性率为20%~90%。除粘液性癌组织中存在HLAI类抗原的低表达外，未发现HLAI类抗原的表达与肿瘤的组织学特征有关。文献报道的粘液性结直肠癌预后较差可能与HLAI类抗原低表达和单核炎性细胞浸润少有关，这可能是对肿瘤细胞免疫反应较弱的反映。
Expression of HLA antigens and beta 2-microglobulin was studied by immunoperoxidase staining of frozen sections of 9 mucinous and 10 nonmucinous colorectal adenocarcinomas, 1 cloacogenic carcinoma, 12 colorectal adenomas and 4 samples of normal colorectal mucosa using monoclonal antibodies (MAbs). Staining results were related to histopathological features. HLA Class I antigens were strongly expressed in morphologically normal colorectal epithelium, in all adenomas tested and in all non-mucinous carcinomas. In contrast, expression of HLA class I antigens by the majority of tumour cells was present in only 2 of the 9 mucinous carcinomas, whereas 2 of these mucinous carcinomas were completely negative. In the mucinous carcinomas a striking scarcity of mononuclear inflammatory infiltrate, especially around the mucus accumulations, was observed. HLA class II antigen expression was not detected in normal epithelium and was only focally present in 1 of the 12 adenomas. In 6 out of the 20 carcinomas tested between 20% and 90% of the tumour cells were stained by MAbs against HLA class II antigens. Apart from the low expression of HLA class I antigens in mucinous carcinomas no relationship was found between expression of HLA antigens and histological features of the tumours. The relative poor prognosis of mucinous colorectal carcinoma as reported in the literature may be associated with low expression of HLA class I antigens and scant mononuclear inflammatory infiltrate, which may be a reflection of a weak immune response to the tumour cells.