Central leptin versus ghrelin: effects on bone marrow adiposity and gene expression
Central leptin versus ghrelin: effects on bone marrow adiposity and gene expression
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DOI：
10.1007/s12020-009-9274-z
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发表时间：
2010-02-01
期刊：
影响因子：
3.7
通讯作者：
Baile, Clifton A.
中科院分区：
文献类型：
--
作者：
Ambati, Suresh;Li, Qiang;Baile, Clifton A.
本研究比较了ghrelin和leptin对身体和骨髓肥胖以及脂肪组织和骨髓中基因表达的中枢作用。雄性Sprague-Dawley大鼠脑室内（ICV）注射对照、66 ng生长素释放肽（G66）、330 ng生长素释放肽（G330）或5 μ g瘦素（L5），每天两次，持续5天。每天测量摄食量（FI）和体重（BW）。采用RT-PCR检测脂肪组织和骨髓中的基因表达。瘦素降低FI（P < 0.05）和BW（P < 0.05），而ghrelin增加BW（P < 0.05），但不影响FI。瘦素降低脂肪垫重量，而ghrelin（G330）增加脂肪垫重量（P < 0.05）。在附睾脂肪组织中，瘦素增加脂解标志物ADRB 2和产热标志物MFN 2的表达，并降低脂肪形成标志物FXR、SLC 2A 4和SCD 1的表达，而ghrelin增加FXR和SCD 1的表达。瘦素减少骨髓脂肪细胞的大小和数量，然而，ghrelin对这些参数没有影响。在全骨髓中，瘦素降低FXR和SCD 1的表达，增加DLK 1的表达，而ghrelin（G330）降低COL 1A 1的表达。因此，瘦素诱导骨髓和脂肪组织基因表达的类似变化，反映了两个区室的肥胖减少。
This study compared the central effects of ghrelin and leptin on body and bone marrow adiposity and gene expression in adipose tissue and bone marrow. Male Sprague-Dawley rats were injected intracerebroventricular (ICV) twice daily with control, 66 ng ghrelin (G66), 330 ng ghrelin (G330), or 5 mu g leptin (L5) for 5 days. Food intake (FI) and body weight (BW) were measured daily. Gene expression in adipose tissue and bone marrow was assessed using RT-PCR. Leptin reduced FI (P < 0.05) and BW (P < 0.05), whereas ghrelin increased BW (P < 0.05) without affecting FI. Leptin decreased fat pad weights, whereas ghrelin (G330) increased fat pad weights (P < 0.05). In epididymal adipose tissue, leptin increased expression of lipolysis marker ADRB2 and thermogenesis marker MFN2 and decreased expression of adipogenic markers, FASN, SLC2A4, and SCD1, whereas ghrelin increased expression of FASN and SCD1. Leptin decreased bone marrow adipocyte size and number; however, ghrelin had no effect on these parameters. In whole bone marrow, leptin decreased expression of FASN and SCD1 and increased expression of DLK1, whereas ghrelin (G330) decreased expression of COL1A1. Thus, leptin induces similar changes in bone marrow and adipose tissue gene expression, reflecting the decreased adiposity in both compartments.