Effects of exercise training and diabetes on cardiac myosin heavy chain composition.
Effects of exercise training and diabetes on cardiac myosin heavy chain composition.
复制标题
运动训练和糖尿病对心肌肌球蛋白重链组成的影响。
DOI：
10.1007/bf00230757
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发表时间：
1992
影响因子：
4.3
通讯作者：
Zhao,J
中科院分区：
文献类型：
--
作者：
Paulson,DJ;Gupta,M;Zak,R;Zhao,J
这项研究确定了先前观察到的运动训练对糖尿病心脏的有益影响是否与心肌肌球蛋白重链(MHC)亚型组成的改变有关。静脉注射诱导大鼠糖尿病模型。链脲佐菌素。训练后的大鼠在跑步机上跑60min/d，27m/min，10%等级。10wk后，用凝胶电泳法分析心肌MHC同工酶蛋白组成。用Northern杂交和狭缝杂交技术检测α-MHC和β-MHC基因的表达。蛋白质和信使核糖核酸分析表明，久坐对照组大鼠表现出α-MHC的优势。久坐的糖尿病患者表现出向β-MHC的转变。运动训练的糖尿病大鼠表现出β-MHC优势。结果表明，糖尿病大鼠的跑台运动训练不能阻止糖尿病引起的MHC组成向β-MHC亚型转变，因此运动训练对糖尿病心脏的益处不太可能是由于肌球蛋白亚型组成的正常化。
This study determined whether the beneficial effects of exercise training on the diabetic heart previously observed are associated with alterations in ventricular myosin heavy chain (MHC) isoform composition. Diabetes was induced in rats by i.v. streptozotocin. Trained rats were run on a treadmill for 60 min/day, 27 m/min, 10% grade. After 10 wks, ventricular MHC isoenzyme protein composition was analyzed for MHC composition using gel electrophoresis. α-MHC and β-MHC mRNA were determined by Northern and slot blot hybridization techniques. Both protein and mRNA analyses indicated that sedentary control rats exhibited a predominance of α-MHC. Sedentary diabetics exhibited a shift to β-MHC. Exercise trained diabetic rats showed a predominance of β-MHC. The results indicate that treadmill exercise training of diabetic rat does not prevent the diabetes-induced shift in MHC composition towards the β-MHC isoform, thus it is unlikely that the beneficial effects of exercise training on the diabetic heart, previously shown, are due to a normalization of the myosin isoform composition.