Quantitative variation of C4 variant proteins associated with many MHC haplotypes.
Quantitative variation of C4 variant proteins associated with many MHC haplotypes.
复制标题
与许多 MHC 单倍型相关的 C4 变异蛋白的数量变异。
DOI：
10.1007/bf02421172
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发表时间：
1989
期刊：
影响因子：
3.2
通讯作者：
Alper,CA
中科院分区：
文献类型：
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作者：
Truedsson,L;Awdeh,Z;Yunis,EJ;Mrose,S;Moore,B;Alper,CA
在64个个体中分析了C4蛋白变异，其中51个为扩展主要组织相容性复合体（MHC）单倍型（MHC等位基因的固定组合）的纯合或杂合。通过对神经氨酸酶和羧肽酶处理样品的染色免疫固定电泳图进行密度扫描，测量每个C4变异的相对数量。C4变异在任何单倍型上的相对浓度都是稳定的，并且在家族中遗传。在研究的8个扩展单倍型中，有5个C4变体的数量相对于相同模式的其他变体增加了：[HLA-B8， SC01， DR3]和[HLA-B7， SC31， DR2]产生了大约两倍的C4B1；[HLA-B18, S042， DR2] C4B2量增加；和[HLA-B44， SC30， DR4]两倍量的C4A3。扩展单倍型[HLA-Bw57， SC61， DR7]产生的C4B1是C4A6的两到三倍。在扩展单倍型[HLA-B44， FC31， DR7]和[HLA-Bw62， SC33， DR4]中，C4同型的表达差异不明显。DNA分析可能只支持单倍型的实际基因重复[HLA-B7， SC31, DR2]。结果表明，除了结构基因数量的变化外，其他mhc相关机制可能参与调节任何单倍型指定的C4A或C4B蛋白的相对数量。
C4 protein variants were analyzed in 64 individuals, of which 51 were either homozygous or heterozygous for an extended major histocompatibility complex (MHC) haplotype (a fixed combination of MHC alleles). The relative amount of each C4 variant was measured by densitometric scanning of stained immunofixed electrophoretic patterns of neuraminidase- and carboxypeptidase-treated samples. The relative concentrations of C4 variants on any haplotype were stable and inherited in families. In five of the eight extended haplotypes investigated, the amount of one of the C4 variants relative to others in the same pattern was increased:[HLA-B8, SC01, DR3]and[HLA-B7, SC31, DR2]produced an approximately doubled amount of C4B1;[HLA-B18, S042, DR2]an increased amount of C4B2; and[HLA-B44, SC30, DR4]a double amount of C4A3. The extended haplotype[HLA-Bw57, SC61, DR7]gave rise to two to three times as much C4B1 as C4A6. In the extended haplotypes[HLA-B44, FC31, DR7]and[HLA-Bw62, SC33, DR4], the results did not clearly indicate differences in expression of the C4 isotypes. DNA analysis possibly supported an actual gene duplication only for the haplotype[HLA-B7, SC31, DR2]. The results suggest that, in addition to variation in the number of structural genes, other MHC-linked mechanisms may be involved in the regulation of the relative amounts of C4A or C4B protein specified by any haplotype.