Non-invasive assessment of positive affective state using infra-red thermography in rats
Non-invasive assessment of positive affective state using infra-red thermography in rats
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DOI：
10.1017/awf.2023.87
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发表时间：
2023-09-29
期刊：
影响因子：
1.2
通讯作者：
Mckeegan，Dorothy E. F.
中科院分区：
文献类型：
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作者：
Wongsaengchan，Chanakarn;Mccafferty，Dominic J.;Mckeegan，Dorothy E. F.
随着近年来动物福利科学对积极福利的关注日益增加，对客观的积极福利指标的需求日益增加。目前尚不清楚体表温度的变化是否可以用于非侵入性地识别和量化哺乳动物的阳性状态。我们用红外热像仪（IRT）连续测量了雄性和雌性Wistar大鼠（Rattus norvegicus）的尾表面温度，并同时观察了它们的行为。如果尾巴表面温度可以区分积极和消极的体验，我们期望与消极体验相比有质的不同反应。给三组大鼠增加食物奖励（中性/无奖励、一奖励和三奖励）。这些老鼠被放置在它们习惯的舞台上，并在接触不同奖励前30秒和后30分钟拍摄。尾温最初从奖励前的基线下降，随后又回到基线温度。这种变化的总体模式与先前研究中受到负面刺激的大鼠相同。然而，尾巴温度的动态变化，特别是恢复速度和行为反应（探索），在中性和奖励大鼠之间有所不同，但没有区分奖励的大小。在热反应和行为反应中发现了性别差异，与奖励大小无关。雌性大鼠表现出比雄性大鼠更大的初始反应和更慢的恢复，强调了在动物福利研究中使用两性的价值。这项研究提高了我们对食物奖励引起的积极情绪对周围体温和行为的影响的理解。
With recent increased focus on positive welfare in animal welfare science, there is demand for objective positive welfare indicators. It is unclear whether changes in body surface temperature can be used to non-invasively identify and quantify positive states in mammals. We recorded continuous measurements of tail surface temperature using infra-red thermography (IRT) and concurrent behavioural observations in male and female Wistar rats (Rattus norvegicus). If tail surface temperature can differentiate between positive and negative experiences, we expect a qualitatively different response compared to negative experiences. Three groups of rats were presented with increasing magnitudes of food rewards (neutral/none, one and three rewards). The rats were placed in an arena to which they were habituated and filmed for 30 s before and 30 min after exposure to different rewards. Tail temperature initially decreased from the pre-reward baseline and subsequently returned towards baseline temperature. The overall pattern of the change was the same as for rats subjected to negative stimuli in previous studies. Nevertheless, dynamic changes in tail temperature, specifically the rate of recovery and the behavioural response (exploration), differed between neutral and rewarded rats but failed to distinguish reward magnitude. Sex differences were found in both thermal and behavioural responses, unrelated to reward magnitudes. Female rats exhibited a greater initial response with a slower recovery than male rats, emphasising the value of using of both sexes in animal welfare research. This study improves our understanding of the effects of positive emotions induced by food reward on peripheral body temperature and behaviour.