Shape and tension distribution of the passive rat diaphragm
Shape and tension distribution of the passive rat diaphragm
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DOI：
10.1152/ajpregu.2001.280.1.r33
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发表时间：
2001-01-01
影响因子：
2.8
通讯作者：
Reid, MB
中科院分区：
文献类型：
--
作者：
Boriek, AM;Rodarte, JR;Reid, MB
我们开发了一种体外制备，以调查在完整的大鼠膈肌的形状和应力分布。我们的假设是隔膜是各向异性的，横向纤维方向的顺应性小于沿着纤维方向的顺应性，因此形状变化可能很小。处死动物（8只大鼠）后，切除整个隔膜并在插入处固定到模具中。充氧的Krebs-Ringer溶液在隔膜下循环并在其表面上灌注。在膈肌表面共缝合20-23个小标记。在渗透压（P-di）为3-15 cm H（2）O时，纤维的横向曲率小于沿着纤维的曲率。使用有限元分析，我们计算膜张力。P-di为15 cmH（2）O时，中央腱张力大于肌肉张力。在肋部区域，最大主张力（σ（1））基本上沿着纤维，并且范围为6-10 g/cm。最小主张力（σ（2））为0.3-4 g/cm。在中央腱中，σ（1）为10-15 g/cm，而σ（2）为4-10 g/cm。横纤维方向上的膜片比沿着纤维方向上的膜片刚度大得多。
We developed an in vitro preparation to investigate shape and stress distribution in the intact rat diaphragm. Our hypothesis was that the diaphragm is anisotropic with smaller compliance in transverse fiber direction than along fibers, and therefore shape change may be small. After the animals were killed (8 rats), the entire diaphragm was excised and fixed into a mold at the insertions. Oxygenated Krebs-Ringer solution was circulated under the diaphragm and perfused over its surface. A total of 20-23 small markers were sutured on the diaphragm surface. At transdiaphragmatic pressure (P-di) of 3-15 cmH(2)O, curvature was smaller in transverse direction than along fibers. Using finite element analysis we computed membrane tension. At P-di of 15 cmH(2)O, tension in central tendon was larger than muscle. In costal region maximum principal tension (sigma (1)) is essentially along the fibers and ranged from 6-10 g/cm. Minimum principal tension (sigma (2)) was 0.3-4 g/cm. In central tendon, sigma (1) was 10-15 g/cm, compared with 4-10 g/cm for sigma (2). The diaphragm was considerably stiffer in transverse fiber direction than along the fibers.