Autophagy in skin wounds: a novel marker for vital reactions
Autophagy in skin wounds: a novel marker for vital reactions
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DOI：
10.1007/s00414-015-1168-4
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发表时间：
2015-05-01
影响因子：
2.1
通讯作者：
Kondo, Toshikazu
中科院分区：
文献类型：
--
作者：
Kimura, Akihiko;Ishida, Yuko;Kondo, Toshikazu
人体尸体机械损伤的活力检测是法医学研究的重要课题之一。为了探索急性机械性创面活力的新标志物，我们对小鼠和人皮肤创面的自噬进行了研究。小鼠皮肤创面的Western blotting分析显示，与未损伤的皮肤组织相比，创面样品中LC3-II显著降低，p62显著增加，创伤后间隔时间为千分之一日元0.5 h。这些观察结果表明，伤口部位的自噬水平降低。在死后伤口样本中，LC3-II和p62水平没有显著变化。此外，死后1 ~ 4天的时间间隔对死前皮肤创面LC3-II和p62的变化无显著影响。与小鼠伤口样品一样，在人皮肤伤口样品中也能检测到LC3-II和p62的这些改变。总的来说，我们对动物和人类样本的研究表明，检测自噬相关分子，如LC3-II和p62，作为伤口活力的标记，可能对法医实践有用。
Detection of vitality of mechanical wounds in human cadavers is one of the important issues in forensic medicine. In order to explore novel markers for vitality of acute mechanical wounds, we investigated autophagy in mouse and human skin wounds. Western blotting analysis of mouse skin wounds showed marked reduction of LC3-II and reciprocal increase of p62 in wound samples with the postinfliction intervals of a parts per thousand yen0.5 h, compared with the uninjured skin tissues. These observations indicated that autophagy level was reduced in the wound sites. In postmortem wound samples, there were no remarkable changes in LC3-II and p62 levels. Furthermore, the postmortem intervals of 1-4 days have no significant effects on the changes of LC3-II and p62 in the antemortem skin wounds. Like murine wound samples, these alterations of LC3-II and p62 could be detected in human skin wound samples. Collectively, our study using animal and human samples implied that the detection of autophagy-related molecules such as LC3-II and p62 might be useful for forensic practice as markers of wound vitality.