NEW EMBEDDING MEDIUM FOR ELECTRON MICROSCOPY
NEW EMBEDDING MEDIUM FOR ELECTRON MICROSCOPY
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DOI：
10.1038/178803a0
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发表时间：
1956-01-01
期刊：
影响因子：
64.8
通讯作者：
GLAUERT, RH
中科院分区：
文献类型：
--
作者：
GLAUERT, AM;ROGERS, GE;GLAUERT, RH
甲基丙烯酸正丁酯和甲基丙烯酸甲酯作为包埋介质1的引入是制备生物材料超薄切片的一个重要发展，但它们的使用伴随着各种主要困难。聚合时会发生收缩，单体无法渗透硬物，因此塑料会收缩，无法提供足够的支撑，有时试样周围会出现无法解释的气泡。此外，聚合过程是不均匀的，有时会导致严重的损坏。这可能在细菌中最明显，因为细菌中通常只剩下空的细胞壁残余。
THE introduction ofn-butyl and methyl meth-acrylates as embedding media1constituted an important development in the preparation of ultra-thin sections of biological material; but their use is attended by various major difficulties. Shrinkage occurs on polymerization, hard objects are not penetrated by the monomer so that the plastic shrinks away and does not provide sufficient support, and sometimes there is an unaccountable appearance of bubbles around the specimen. Moreover, the polymerization process is not uniform and sometimes causes severe damage. This is perhaps most strikingly seen in bacteria where often only empty remnants of cell walls remain.