Generation of integration-free neural progenitor cells from cells in human urine
Generation of integration-free neural progenitor cells from cells in human urine
复制标题
从人尿液中的细胞生成无整合的神经祖细胞
DOI：
10.1038/nmeth.2283
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发表时间：
2013-01-01
期刊：
影响因子：
48
通讯作者：
Pei, Duanqing
中科院分区：
文献类型：
--
作者：
Wang, Lihui;Wang, Linli;Pei, Duanqing
人类神经干细胞在神经系统疾病的研究和治疗中具有巨大的前景。我们描述了无整合和可扩增的人神经祖细胞（NPC）的产生。我们结合了一个附加型系统，以提供重编程因子与化学成分确定的培养基，以重编程上皮样细胞从人尿成NPC（hUiNPC）。这些无转基因hUiNPC可以自我更新，并可以在体外分化为多种功能的神经元亚型和胶质细胞。虽然功能活体分析仍然需要，我们报告说，细胞存活和分化后移植到新生大鼠脑。
Human neural stem cells hold great promise for research and therapy in neural disease. We describe the generation of integration-free and expandable human neural progenitor cells (NPCs). We combined an episomal system to deliver reprogramming factors with a chemically defined culture medium to reprogram epithelial-like cells from human urine into NPCs (hUiNPCs). These transgene-free hUiNPCs can self-renew and can differentiate into multiple functional neuronal subtypes and glial cellsin vitro. Although functionalin vivoanalysis is still needed, we report that the cells survive and differentiate upon transplant into newborn rat brain.