Auxin transport inhibitors block PIN1 cycling and vesicle traficking
Auxin transport inhibitors block PIN1 cycling and vesicle traficking
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DOI：
10.1038/35096571
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发表时间：
2001-09-27
期刊：
影响因子：
64.8
通讯作者：
Palme, K
中科院分区：
文献类型：
--
作者：
Geldner, N;Friml, J;Palme, K
植物激素生长素的极性运输介导植物生长和发育中的各种过程，例如顶端优势、向性、维管模式和轴形成（1，2）。这种观点主要是基于极性生长素运输抑制剂的影响。这些化合物破坏生长素从细胞中流出，但其作用方式尚不清楚（3）。据认为，极性生长素通量是由作用于质膜的外排载体的不对称分布引起的（4）。外排载体候选PIN 1的极性定位支持该模型（4）。在这里，我们表明，看似静态的本地化的PIN 1的结果从快速肌动蛋白依赖性细胞质膜和内体室之间的循环。生长素转运抑制剂阻断PIN1循环并抑制与生长素转运无关的膜蛋白的运输。我们的数据表明，PIN1循环是生长素运输的核心重要性，生长素运输抑制剂通常通过干扰膜运输过程来影响外排。为了支持我们的结论，囊泡运输抑制剂布雷菲德菌素A模拟生长素运输抑制剂的生理作用。
Polar transport of the phytohormone auxin mediates various processes in plant growth and development, such as apical dominance, tropisms, vascular patterning and axis formation(1,2). This view is based largely on the effects of polar auxin transport inhibitors. These compounds disrupt auxin efflux from the cell but their mode of action is unknown(3). It is thought that polar auxin flux is caused by the asymmetric distribution of efflux carriers acting at the plasma membrane(4). The polar localization of efflux carrier candidate PIN1 supports this model(4). Here we show that the seemingly static localization of PIN1 results from rapid actin-dependent cycling between the plasma membrane and endosomal compartments. Auxin transport inhibitors block PIN1 cycling and inhibit trafficking of membrane proteins that are unrelated to auxin transport. Our data suggest that PIN1 cycling is of central importance for auxin transport and that auxin transport inhibitors affect efflux by generally interfering with membrane-trafficking processes. In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors.