Three Types of Acidic Polysaccharides Associated with Coccolith of Pleurochrysis haptonemofera: Comparison with Pleurochrysis carterae and Analysis Using Fluorescein-Isothiocyanate-Labeled Lectins
Three Types of Acidic Polysaccharides Associated with Coccolith of Pleurochrysis haptonemofera: Comparison with Pleurochrysis carterae and Analysis Using Fluorescein-Isothiocyanate-Labeled Lectins
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与侧金藻球藻相关的三种酸性多糖：与侧金藻的比较以及使用荧光素-异硫氰酸酯标记的凝集素进行分析
DOI：
10.1007/s10126-004-5148-9
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发表时间：
2005
影响因子：
3
通讯作者：
M. Tsuzuki
中科院分区：
文献类型：
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作者：
Yasutaka Hirokawa;S. Fujiwara;M. Tsuzuki
在颗石藻类微藻中，酸性多糖被认为参与了钙化鳞片（颗石）的形成。对球石藻类半触片侧金藻细胞表面酸性多糖的性质进行了分析。细胞表面的酸性多糖可以通过荧光素-异硫氰酸酯标记的凝集素染色后用流式细胞仪测量细胞的荧光来检测。流式细胞仪分析表明，酸性多糖仍然在细胞表面上，即使在CaCO 3的颗石被溶解后，通过降低pH值，但他们被提取，通过随后的EDTA或EGTA处理，这表明他们是绑定到CaCO 3晶体的颗石，但通过Ca 2+到表面上。通过阴离子交换层析、二价阳离子胶体沉淀和聚丙烯酰胺凝胶电泳（PAGE）对酸性多糖进行分析，结果表明，该菌含有3种类型的酸性多糖（Ph-PS-1、-2和-3）。PAGE图谱表明，Ph-PS-2具有重复结构，分子量范围较宽，如侧鞭金藻（Pleurochrysiscarterae），而Ph-PS-1和Ph-PS-3分别含有几个次要组分和一个主要组分。Ph-PS-1和Ph-PS-3中未发现的微量成分可能是P. haptonemofera的特征成分。用不同浓度的EDTA和EGTA处理的细胞表面和提取物的分析表明，Ph-PS-2，这是由一个更高的亲和力，刀豆球蛋白A区分，结合到颗石表面上更强烈比其他两种类型的酸性多糖。
In the coccolithophorid microalgae acidic polysaccharides are considered to be involved in the formation of the calcified scale, coccolith. Characteristics of the acidic polysaccharides extracted from the cell surface of the coccolithophorid Pleurochrysis haptonemofera were analyzed. The acidic polysaccharides on the cell surface can be detected by measuring fluorescence of cells after fluorescein-isothiocyanate-labeled lectin staining by flow cytometry. Flow cytometric analyses revealed that the acidic polysaccharides remained on the cell surface even after CaCO3 in the coccolith was dissolved by lowering pH, but they were extracted by subsequent EDTA or EGTA treatment, suggesting that they are bound not into the CaCO3 crystals of the coccolith, but onto the surface via Ca2+. Analyses of the acidic polysaccharides by anion exchange chromatography, colloidal precipitation with divalent cations, and polyacrylamide gel electrophoresis (PAGE) revealed that P. haptonemofera has 3 types of acidic polysaccharides (Ph-PS-l, -2, and -3). The PAGE patterns suggested that Ph-PS-2 has a repeated structure with a broad range of molecular weight, as in Pleurochrysis carterae, while Ph-PS-1 and -3 contain several minor components in addition to a major component, respectively. The minor components in Ph-PS-1 and -3 that have not been found in P. carterae might be characteristic of P. haptonemofera. Analyses of both the cell surface treated by various concentrations of EDTA and EGTA and the extracts suggested that Ph-PS-2, which is distinguishable by a higher affinity to concanavalin A, is bound onto the coccolith surface more intensely than the other two types of acidic polysaccharides.
影响因子：
--
作者：
MARSH, ME
通讯作者：
MARSH, ME
从单细胞颗石藻类 Pleurochrysis carterae 的矿化鳞片中分离和表征含有酒石酸盐和乙醛酸盐残留物的新型酸性多糖。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Marsh,ME;Chang,DK;King,GC
通讯作者：
King,GC