Comparison of methylation-specific polymerase chain reaction (MSP) with reverse transcriptase-polymerase chain reaction (RT-PCR) in peripheral blood of gastric cancer patients
Comparison of methylation-specific polymerase chain reaction (MSP) with reverse transcriptase-polymerase chain reaction (RT-PCR) in peripheral blood of gastric cancer patients
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DOI：
10.1002/jso.20106
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发表时间：
2004-09-15
影响因子：
2.5
通讯作者：
Yamagishi, H
中科院分区：
文献类型：
--
作者：
Koike, H;Ichikawa, D;Yamagishi, H
背景和目的：为了实现胃癌的完全治愈，必须在早期发现原发性和复发性肿瘤。本研究旨在比较胃癌患者血液标本中p16、E-cadherin和RAR β基因的癌胚抗原（CEA）特异性逆转录聚合酶链反应（RT-PCR）和甲基化特异性聚合酶链反应（MSP）。41例胃癌患者（包括9例早期胃癌患者）术前采集血样，进行RT-PCR和MSP检测。41例患者中有10例（24%）通过RTLPCR显示CEA特异性信号。I、II、III和IV期的阳性率分别为11%、13%、50%和50%。RT-PCR结果与分期有显著相关性（P < 0.01）。MSP检测在9例患者（22%）中检测到p16的高甲基化，在9例患者（22%）中检测到E-钙粘蛋白，在6例患者（15%）中检测到RAR β。共有18例患者（44%）表现出高甲基化。Ⅰ、Ⅱ、Ⅲ、Ⅳ期阳性率分别为37%、50%、40%、75%。异常甲基化与静脉浸润密切相关（P < 0.05）。结论：MSP在胃癌中的检出率高于RT-PCR。这两种检测都可以作为标记物，允许选择需要更密集的筛查和积极的术后治疗的病例。(C)2004 Wiley-Liss，Inc.
Background and Objectives: To achieve a complete cure in gastric cancer, primary and recurrent tumors must be detected at an early stage. This study was designed to compare carcinoembryonic antigen (CEA)-specific reverse transcriptase-polymerase chain reaction (RT-PCR) and methylation-specific polymerase chain reaction (MSP) for p16, E-cadherin, and retinoic acid receptor beta (RARbeta) genes using blood samples from gastric cancer patients.Methods: Preoperative blood samples obtained from 41 patients with gastric cancer, including 9 with early-stage disease, and were subjected to RT-PCR and MSP assays.Results: Ten of 41 (24%) patients exhibited a CEA-specific signal by RTLPCR. Positive rates were 11, 13, 50, and 50% in stages I, II, III, and IV, respectively. A significant association was found between RT-PCR results and stage (P < 0.01). The MSP assay detected hypermethylation of p16 in 9 patients (22%), E-cadherin in 9 patients (22%), and RARbeta in 6 patients (15%). Altogether, 18 patients (44%) showed hypermethylation. The positive rates were 37, 50, 40, and 75% in stages I, II, III, and IV, respectively. A significant association was found between aberrant methylation and venous invasion (P < 0.05). Neither the CEA-specific signal nor hypermethylation was detected in serum from control volunteers.Conclusions: The detection rate of MSP was higher than that of RT-PCR in gastric cancer. Both assays can serve as markers that allow selection of those cases requiring more intensive screening and aggressive postoperative treatment. (C) 2004 Wiley-Liss, Inc.