Permeability and vascularity of the developing brain: Cerebellum vs cerebral cortex
Permeability and vascularity of the developing brain: Cerebellum vs cerebral cortex
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发育中大脑的渗透性和血管分布：小脑与大脑皮层
DOI：
10.1016/0006-8993(80)91155-5
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
C. Johanson
中科院分区：
文献类型：
--
作者：
C. Johanson
利用不同出生后年龄的大鼠研究了[~ 3 H]甘露醇、[~ 3 H]菊粉和[~（51）Cr]标记的红细胞在小脑（CER）和大脑皮质（CC）中短期分布的发育变化。随着CNS的成熟，CER中1-h放射性甘露醇（和菊粉）空间的最急剧减少发生在出生后第二周，而CC中两种放射性同位素空间的最大减少发生在0.5至1周的早期。[3 H]甘露醇在1小时内渗透到未成熟脑组织（1周）中的分布体积显著小于（75-90%）该示踪剂渗透到非神经组织（如心脏和肝脏）中的分布体积;因此，即使在神经胶质细胞增殖之前，也存在有限的屏障效应。第3周时，CER中的血脑屏障对甘露醇的渗透性与CC相当。CER的血管分布（根据残留[51 Cr]红细胞体积估计）大于研究的每个出生后年龄的CC。未成熟动物脉络丛（血-CSF屏障）对放射性甘露醇的摄取主要反映了细胞外液体积的发育变化，而不是脉络膜上皮基底侧面渗透性的变化。新生儿与成人脑脊液和脑组织之间[3 H]甘露醇分布的定性差异归因于与血-脑脊液和血脑屏障相关的分泌和渗透性现象的成熟变化。
Rats of various postnatal ages were utilized to investigate developmental changes in the short-term distribution of [3H]mannitol, [3H]inulin and [51Cr]-tagged erythrocytes in both the cerebellum (CER) and cerebral cortex (CC). As the CNS undergoes maturation the most precipitous decrease in the 1-h radiomannitol (and inulin) space in the CER takes place during the second postnatal week ehereas the greatest reduction in the spaces for both radioisotopes in the CC occurs during the earlier period between 0.5 and 1 week. The volume of distribution in immature brain tissue (1 week) into which [3H]mannitol penetrates in 1 h is substantially less (by 75–90%) than that into which this tracer permeates in non-neural tissue such as heart and liver; thus, even prior to the proliferation of glial elements, there is a limited barrier effect. At 3 weeks the permeability of the blood-brain barrier in the CER to mannitol is comparable to that in the CC. The vascularity of the CER (as estimated by residual [51Cr]erythrocyte-volume) is greater than that of the CC at each postnatal age investigated. The uptake of radiomannitol by the choroid plexus (blood-CSF barrier) in immature animals is a reflection primarily of development l changes in volume of extracellular fluid rather than in permeability of the basolateral face of the choroidal epithelium. Qualitative differences, neonates vs adults, in the distribution of [3H]mannitol between CSF and brain tissue are attributable to maturational changes in secretory and permeability phenomena associated with the blood-CSF and blood-brain barriers.