Near-infrared diffuse in vivo flow cytometry.
Near-infrared diffuse in vivo flow cytometry.
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DOI：
10.1117/1.jbo.27.9.097002
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发表时间：
2022-09
影响因子：
3.5
通讯作者：
中科院分区：
文献类型：
--
作者：
弥漫性活体流式细胞术(DIFC)是一种新兴的技术，可以无创地计数血流中罕见的荧光标记循环细胞。到目前为止，我们已经报道了红色和蓝绿色版本的DIFC。使用近红外(NIR)荧光灯原则上将允许在更深的组织中使用DIFC，并将与新兴的近红外荧光分子造影剂兼容。我们描述了一种NIR-DIFC仪器的设计，并演示了它在体外和体内小鼠的光流模型中的应用。我们开发了一种改进的光纤探头设计，可以有效地收集单个循环细胞的荧光，并有效地抑制仪器的自身荧光。我们建造了一台NIR-DIFC仪器。我们用近红外荧光微球和标记了OTL38荧光造影剂的细胞系在流动模型中测试了这一点。我们还在静脉注射OTL38标记的L1210A细胞的裸鼠身上测试了NIR-DIFC。NIR-DIFC可以检测到流动模体中的循环肿瘤细胞(CTC)，平均信噪比(SNR)为19到32分贝。在小鼠中，荧光标记的CTCs可被检测到，平均信噪比为2600db。NIR-DIFC也表现出明显低于蓝绿色DIFC的自发荧光和错误报警率。NIR-DIFC允许使用新兴的近红外造影剂。我们的工作可能为NIR-DIFC未来在人类身上的应用铺平道路。
Diffuse in vivo flow cytometry (DiFC) is an emerging technique for enumerating rare fluorescently labeled circulating cells noninvasively in the bloodstream. Thus far, we have reported red and blue-green versions of DiFC. Use of near-infrared (NIR) fluorescent light would in principle allow use of DiFC in deeper tissues and would be compatible with emerging NIR fluorescence molecular contrast agents. We describe the design of a NIR-DiFC instrument and demonstrate its use in optical flow phantoms in vitro and in mice in vivo. We developed an improved optical fiber probe design for efficient collection of fluorescence from individual circulating cells and efficient rejection of instrument autofluorescence. We built a NIR-DiFC instrument. We tested this with NIR fluorescent microspheres and cell lines labeled with OTL38 fluorescence contrast agent in a flow phantom model. We also tested NIR-DiFC in nude mice injected intravenously with OTL38-labeled L1210A cells. NIR-DiFC allowed detection of circulating tumor cells (CTCs) in flow phantoms with mean signal-to-noise ratios (SNRs) of 19 to 32 dB. In mice, fluorescently labeled CTCs were detectable with mean SNR of 26 dB. NIR-DiFC also exhibited orders significantly lower autofluorescence and false-alarm rates than blue-green DiFC. NIR-DiFC allows use of emerging NIR contrast agents. Our work could pave the way for future use of NIR-DiFC in humans.
影响因子：
3.5
作者：
Zettergren E;Swamy T;Runnels J;Lin CP;Niedre M
通讯作者：
Niedre M