Genetic and environmental effects on the growth and differentiation of wheat somatic cell cultures
Genetic and environmental effects on the growth and differentiation of wheat somatic cell cultures
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DOI：
10.1093/oxfordjournals.jhered.a109809
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发表时间：
1983-09
影响因子：
3.1
通讯作者：
M. Lazar;G. Collins;W. E. Vian
中科院分区：
文献类型：
--
作者：
M. Lazar;G. Collins;W. E. Vian
以小麦（Triticum aestivumL.） 25个品种和另外5个品种的成熟胚和未成熟胚为材料，在多种环境条件下进行体外培养。愈伤组织增殖率在不同基因型、不同激素来源和浓度之间存在显著差异。愈伤组织增殖的最佳培养基为0.5 ~ 1.0 mg I−12,4,5- t和0.1 mg I−1kinetin。芽分化发生的频率较低（≤3%的应答菌落），但因基因型而有显著差异。胚培养再生的2个愈伤组织的增殖速度均不高于亲本品种，但愈伤组织增殖的系内变异率明显低于亲本品种。其中一个胚培养衍生系比亲本品种分化芽的频率更高。愈伤组织生长和分化的组织学检查有力地支持了愈伤组织内部新生的分生组织产生芽分化的假说。
Mature and immature embryos of 25 wheat (Triticum aestivumL.) cultivars and five additionalTriticumspecies were cultured in vitro under a wide variety of environmental conditions. Callus proliferation rate varied significantly among genotypes and among phytohormone sources and concentrations. Optimum medium for callus proliferation contained 0.5–1.0 mg I−12,4,5-T and 0.1 mg I−1kinetin. Shoot differentiation occurred at low frequency (≤3 percent of colonies responding), but varied significantly with genotype. Two lines derived by regeneration from embryo culture proliferated in culture no faster than their respective parent cultivars, although the within-line variability for callus proliferation was significantly less than the variability within parent cultivars. One of the embryoculture derived lines differentiated shoots more frequently than the parent cultivar. Histological examination of calli undergoing growth and differentiation strongly supported the hypothesis that shoot differentiation derived from meristems formed de novo within the callus.