Evaluation of the Ability of Malassezia Species in Biofilm Formation
Evaluation of the Ability of Malassezia Species in Biofilm Formation
复制标题
马拉色菌生物膜形成能力的评价
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Zeinab Borjian Borujeni
中科院分区：
文献类型：
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作者：
M. Zareei;S. Mohammadi;S. Shahbazi;M. Roudbary;Zeinab Borjian Borujeni
背景：虽然属马拉色菌是皮肤正常菌群的一部分，但在一定条件下，它们会变成致病性的。由马拉色菌引起的导管相关性真菌病与生物膜形成有关，被认为是一种疾病感染。目的：本研究的目的是评价球形马拉色菌和限制性马拉色菌在生物膜形成中的能力。方法：采用导管段在12孔板上形成生物膜。采用96孔板3-（4,5 -二甲基噻唑-2-基）- 2,5 -二苯基溴化四唑（MTT）比色法测定结果。数据分析采用单变量方差分析（ANOVA）或重复测量方差分析（Repeated Measures ANOVA）。P值≤0.05认为有统计学意义。采用IBM SPSS Statistics version 22.0软件进行统计分析。结果：球形芽孢杆菌和限制性芽孢杆菌均能在体外形成生物膜。限制马拉色菌比球形马拉色菌更能形成生物膜，但差异无统计学意义（P = 0.192）。结论：随着时间的推移，马拉色菌生物膜逐渐成熟。由于上述物种形成生物膜的能力，它们可能在真菌病中发挥重要作用，应在治疗过程中予以考虑。
Background: Although Malassezia genusare part of the skin normal flora, under certain conditions, they become pathogenic. Catheter-related fungemia, caused by Malassezia , whichis associated with biofilmformation, is considered anosocomial infection. Objectives: The aim of this study was to evaluate the ability of Malassezia globosa and Malassezia restricta in biofilm formation. Methods: Biofilm formation was carried out using catheter segments in 12-well plates. Results were measured using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) colorimetric assay in 96-well plates. The data was analyzed using univariate Analysis of Variance (ANOVA) or Repeated Measures ANOVA. P values of ≤ 0.05 were considered statistically significant. Statistical analyses were performed using IBM SPSS Statistics version 22.0 software. Results: Both M. globosa and M. restricta species were able to form biofilms in vitro. Malassezia restricta was more capable than M. globosa to form biofilms, yet, significant differences were not observed (P = 0.192). Conclusions: Over time, Malassezia biofilms matured. Due to the above species ability in forming biofilm, they could play an important role in fungemia that should be considered in therapeutic procedures.