ROLE OF LAMININ A-CHAIN IN THE DEVELOPMENT OF EPITHELIAL-CELL POLARITY
ROLE OF LAMININ A-CHAIN IN THE DEVELOPMENT OF EPITHELIAL-CELL POLARITY
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DOI：
10.1016/0092-8674(88)90056-6
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发表时间：
1988-10-21
期刊：
影响因子：
64.5
通讯作者：
EKBLOM, P
中科院分区：
文献类型：
--
作者：
KLEIN, G;LANGEGGER, M;EKBLOM, P
Kidney organ culture was used to study the conversion of embryonic mesenchymal cells into a polarized, differentiated kidney epithelium. We examined the expression of laminin, a basement membrane glycoprotein, during this conversion. The B chains of laminin were constitutively expressed, whereas the appearance of the A chain of laminin was dependent on embryonic induction and coincided with the onset of cell polarization. Antisera against the carboxy-terminal end of laminin inhibited polarization but did not affect the developmental events that precede polarization. Antisera against N-terminal parts of laminin failed to inhibit morphogenesis. Since the fragments at the carboxy-terminal end contain parts of the A chain, we suggest that the appearance of this chain is fundamental for initiation of cell polarity.
