Anti-proliferative and apoptosis-inducible activity of labdane and abietane diterpenoids from the pulp of Torreya nucifera in HeLa cells
Anti-proliferative and apoptosis-inducible activity of labdane and abietane diterpenoids from the pulp of Torreya nucifera in HeLa cells
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DOI：
10.3892/mmr_00000315
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发表时间：
2010-07-01
影响因子：
3.4
通讯作者：
Suzuki, Nobuo
中科院分区：
文献类型：
--
作者：
Chen, Shi-Ping;Dong, Mei;Suzuki, Nobuo
从香榧果肉的甲醇提取物中分离得到两个松香烷型和一个唇形科二萜类化合物，并研究了它们对人癌细胞生长的抑制作用。在这三种化合物中，实验室化合物kayadiol被发现对多种人类癌细胞具有最有效的抑制作用。使用MTT测定法，确定kayadiol在HeLa细胞中具有30 μ M的IC 50（50%抑制浓度），并且还对六种其它人类癌细胞系显示出抗增殖作用，IC 50值为30-50 μ M。Kayadiol处理HeLa细胞导致剂量依赖性的凋亡事件的产生，包括DNA梯状（
Two abietane and one labdane type diterpenoids were isolated from the methanol extracts of Torreya nucifera pulp and investigated for their ability to inhibit the growth of human cancer cells. Among the three compounds, the labdane compound kayadiol was found to have the most effective inhibitory effect against a wide variety of human cancer cells. Using the MTT assay, kayadiol was determined to have an IC50 (50% inhibition concentration) of 30 mu M in HeLa cells, and also to exhibit anti-proliferative effects towards six other human cancer cell lines, with IC50 values of 30-50 mu M. Kayadiol treatment of HeLa cells resulted in a dose-dependent generation of apoptotic events, including DNA laddering (