Mucosa-associated bacteria in ulcerative colitis before and after antibiotic combination therapy
Mucosa-associated bacteria in ulcerative colitis before and after antibiotic combination therapy
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DOI：
10.1111/j.1365-2036.2005.02428.x
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发表时间：
2005-04-15
影响因子：
7.6
通讯作者：
Sato, N
中科院分区：
文献类型：
--
作者：
Nomura, T;Ohkusa, T;Sato, N
背景资料：目的：探讨抗生素联合治疗对溃疡性结肠炎的疗效。方法：将20例溃疡性结肠炎患者随机分为两组，抗生素治疗组（阿莫西林、四环素、甲硝唑治疗2周）和非抗生素治疗组。在治疗后0个月和3-5个月进行临床评估、结肠镜和组织学评估。从活检标本中分离粘膜细菌的DNA。我们调查了粘膜相关的细菌成分的限制性内切酶HhaI和MspI的末端限制性片段长度多态性，并通过实时聚合酶链反应定量的细菌数量的变化。免疫组化检测F.结果：抗生素组治疗后临床评分、结肠镜评分及组织学评分均较对照组改善。三个末端限制性片段长度多态性峰仅在抗生素组治疗后减少。直肠真杆菌（Eubacterium rectale）、产蚁多膜菌（Dorea formicigenans）、梭状芽孢杆菌（Clostridium clostridioforme）和F.变异包括在这些峰中。基于实时荧光PCR技术的研究，只有F。治疗后变异明显减少。结论：抗生素联合治疗溃疡性结肠炎疗效确切，且安全性高。粘膜相关F.治疗后变异显著降低。
Background: We proposed that Fusobacterium varium is one of the causative agents in ulcerative colitis.Aim: To examine the efficacy of antibiotic combination therapy against F. varium and to investigate the mucosa-associated bacteria before and after the therapy using a new molecular approach.Methods: Twenty patients with ulcerative colitis were randomly assigned into the antibiotic treatment group (amoxicillin, tetracycline and metronidazole for 2 weeks) and no-antibiotics group. Clinical assessment, colonoscopic and histological evaluations were performed at 0 and 3-5 months after the treatment. DNA from mucosal bacteria was isolated from biopsy specimens. We investigated the mucosa-associated bacterial components by terminal restriction fragment length polymorphism with the restriction enzyme HhaI and MspI, and quantified the change in the number of bacteria by real-time polymerase chain reaction. Immunohistochemical detection of F. varium in biopsy specimens was also performed.Results: After the treatment, the clinical assessment, colonoscopic and histological scores improved in the antibiotic group compared with the control group. Three peaks of terminal restriction fragment length polymorphism decreased after treatment only in the antibiotic group. Eubacterium rectale, Dorea formicigenerans, Clostridium clostridioforme and F. varium were included in these peaks. Based on the real-time polymerase chain reaction study, only F. varium was significantly reduced after treatment. In the immunostaining, post-treatment scores in treatment group were significantly lower than that in control group.Conclusions: Antibiotics combination therapy was effective for ulcerative colitis. The number of mucosa-associated F. varium significantly decreased after the treatment.